Brain stem global gene expression profiles in human spina bifida embryos  

作　　者：Hong Zhao Xiang Li Wan-I Li Quanren He Ting Zhang Xiaoying Zheng Ran Zhou JunXie 

机构地区：[1]Department of Biochemistry and Molecular Biology, Ministry of Education Key Laboratory of Cellular Physiology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China [2]Department of Physiology and Pharmacology, University of Georgia, Georgia 361004, USA [3]Capital Institute of Pediatrics, Beijing 100020, China [4]Institute of Population Research, Peking University, Beijing 100732, China [5]Shanxi Traditional Medical College, Taiyuan 030024, Shanxi Province, China

出　　处：《Neural Regeneration Research》2011年第5期366-372,共7页中国神经再生研究（英文版）

基　　金：Supported by the National Key Project of Scientific and Technical Supporting Programs funded by the Ministry of Science & Technology of China, No. 2007BA107A02;the National Basic Research Program of China (973 Program), No. 2007CB511902;the Shanxi Scholarship Council of China, No. 2008-48;the Shanxi Natural Science Foundation, No. 2010011049-2;the National Natural Science Foundation of China, No. 31040056

摘　　要：Environmental and genetic factors influence the occurrence of neural tube defects, such as spina bifida. Specific disease expression patterns will help to elucidate the pathogenesis of disease. However, results obtained from animal models, which often exhibit organism specificity, do not fully explain the mechanisms of human spina bifida onset. In the present study, three embryos with a gestational age of approximately 17 weeks and a confirmed diagnosis of spina bifida, as well as 3 age-matched normal embryos, were obtained from abortions. Fetal brain stem tissues were dissected for RNA isolation, and microarray analyses were conducted to examine profiles of gene expression in brain stems of spina bifida and normal embryos using Affymetrix HG-U133A 2.0 GeneChip arrays. Of the 14 500 gene transcripts examined, a total of 182 genes exhibited at least 2.5-fold change in expression, including 140 upregulated and 42 downregulated genes. These genes were placed into 19 main functional categories according to the Gene Ontology Consortium database for biological functions. Of the 182 altered genes, approximately 50% were involved in cellular apoptosis, growth, adhesion, cell cycle, stress, DNA replication and repair, signal transduction, nervous system development, oxidoreduction, immune responses, and regulation of gene transcription. Gene expression in multiple biological pathways was altered in the brain stem of human spina bifida embryos.Environmental and genetic factors influence the occurrence of neural tube defects, such as spina bifida. Specific disease expression patterns will help to elucidate the pathogenesis of disease. However, results obtained from animal models, which often exhibit organism specificity, do not fully explain the mechanisms of human spina bifida onset. In the present study, three embryos with a gestational age of approximately 17 weeks and a confirmed diagnosis of spina bifida, as well as 3 age-matched normal embryos, were obtained from abortions. Fetal brain stem tissues were dissected for RNA isolation, and microarray analyses were conducted to examine profiles of gene expression in brain stems of spina bifida and normal embryos using Affymetrix HG-U133A 2.0 GeneChip arrays. Of the 14 500 gene transcripts examined, a total of 182 genes exhibited at least 2.5-fold change in expression, including 140 upregulated and 42 downregulated genes. These genes were placed into 19 main functional categories according to the Gene Ontology Consortium database for biological functions. Of the 182 altered genes, approximately 50% were involved in cellular apoptosis, growth, adhesion, cell cycle, stress, DNA replication and repair, signal transduction, nervous system development, oxidoreduction, immune responses, and regulation of gene transcription. Gene expression in multiple biological pathways was altered in the brain stem of human spina bifida embryos.

关 键 词：APOPTOSIS GENECHIP gene expression neural tube defect spina bifida 

分 类 号：Q786[生物学—分子生物学] Q987