低频超声波对双氯芬酸钠体外经皮渗透的影响  

Effect of low frequency ultrasound on percutaneous absorption of diclofenac sodium in vitro

作　　者：胡志刚[1] 王新征[1] 冯坤 介晓阳[3] 

机构地区：[1]河南科技大学机电工程学院,河南省洛阳市471003 [2]河南洛阳正骨医院研究院实验中心,河南省洛阳市471000 [3]河南科技大学医学技术工程学院,河南省洛阳市471003

出　　处：《中国组织工程研究与临床康复》2011年第8期1429-1432,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家高技术研究发展计划(863计划)(2008AA040208);课题名称:个人卫生护理机器人~~

摘　　要：背景:双氯芬酸钠是临床上常用的强效非类固醇抗炎镇痛药物,经皮渗透可以避免肝的首过作用和胃肠道破坏,但受角质层影响,渗透量小;低频超声波能够改善皮肤通透性,促进药物经皮吸收。目的:通过对低频超声对促进双氯芬酸钠药物经皮的吸收效果研究,确定适合该类药物的低频超声经皮渗透物理参数。方法:30只大鼠随机均分为10组,9组用于正交试验,1组用于不加超声波时的对照试验。取大鼠背部皮肤进行透皮实验,以超声波的频率、强度及作用时间为影响因素,渗透量为指标,采用正交试验法,对双氯芬酸钠进行大鼠离体皮肤体外渗透试验,考察双氯芬酸钠渗透效果最好时,低频超声波各个参数值最优配比,并与空白组对比,观察其显著意义。结果与结论:渗透量随着超声波的作用时间的延长而增加,且超声波频率和强度都对渗透量有一定的影响。正交试验筛选的低频超声波最佳配比的参数为频率20kHz,强度0.75W/cm2,作用时间15min。结果提示,低频超声波频率、强度、作用时间均对渗透量的影响显著(P<0.01),三者相比较,作用时间对双氯芬酸钠渗透性的影响最大。BACKGROUND：Diclofenac sodium is common used on clinical as a powerful non-steroid anti inflammatory and antalgic drug.Gastrointestinal tract degradation and first-pass effect could be avoided after transdermal delivery.But because of the barrier of skin,transdermal drug delivery is limited.The application of low-frequency ultrasound can promote the drug percutaneous absorption by increasing the permeability of the skin.OBJECTIVE：Based on the study of the influence on percutaneous absorption of diclofenac sodium by low frequency ultrasound,the optimum parameters of diclofenac sodium under low frequency ultrasound were determined.METHODS：Thirty male rats were randomly divided into ten groups,nine of them were used for orthogonal experiment while one for control experiment without ultrasound.The backside skins of rats were selected for transdermal test.The orthogonal experiment was performed in vitro with ultrasonic frequency,intensity and duration as influencing factors,penetration amount as index,and each parameter of low frequency ultrasound was optimized at the optimal permeation effect of diclofenac sodium,and compared with blank group to observe its significance.RESULTS AND CONCLUTION：Ultrasonic intensity,frequency,especially duration had very important influence on the increase in the penetration amount.The optimized parameter combination obtained by orthogonal design was composed of ultrasonic frequency（20 kHz）,intensity（0.75 W/cm2） and duration（15 minutes）.The frequency,intensity,duration of low frequency ultrasound have a significant effect on the penetration amount of diclofenac sodium（P 0.01）.By the comparison of three parameters,ultrasonic duration is the most important.

关 键 词：低频超声波 经皮渗透 双氯芬酸钠 强度 作用时间 

分 类 号：R318[医药卫生—生物医学工程]