实验性大鼠脑震荡后c-jun mRNA表达  

A study on the expression of c-jun mRNA after experimental rat brain concussion

作　　者：汪枫[1] 李永宏[1] 

机构地区：[1]皖南医学院法医系,安徽芜湖241001

出　　处：《中国法医学杂志》2011年第1期10-12,I0002,共4页Chinese Journal of Forensic Medicine

摘　　要：目的观察实验性大鼠脑震荡后c-jun mRNA的表达变化规律。方法 55只实验大鼠随机分为脑震荡组(0min、15min、30min、60min、3h、6h、12h、24h、48h、96h)和对照组,用原位杂交法观察大鼠脑震荡后各时间点,大脑皮质、脑干和丘脑神经元c-jun mRNA表达的变化规律。结果对照组大鼠神经元和胶质细胞均可见c-jun mRNA的弱阳性表达。脑震荡组大鼠损伤后15min神经细胞观察到c-jun mRNA阳性表达,随损伤后经过时间的延长阳性表达逐渐增强;30min时c-jun mRNA阳性反应达高峰,随后逐渐降低,至96h时回落至对照组水平。结论 c-jun mRNA表达水平可成为诊断脑震荡和推断伤后经过时间的一项敏感指标。Objective To evaluate changes of c-jun mRNA after brain concussion.Methods Fifty-five rats were randomly divided into brain concussion groups（0min,15min,30min,60min,3h,6h,12h,24h,48h,96h） and control group.The expression of c-jun mRNA in cortex、thalamus and brain stem was microscopically observed by In site hybridization method.Results There were weak positive expression of c-jun mRNA in some neutrons and neuroglia cells in control group.In brain concussion group,however,positive expression of c-jun mRNA in some neutrons was seen at 15min after brain concussion,and reach to the peak at 30min after brain concussion the level of expression of c-jun mRNA were as well as control group at 96h.Conclusion There findings suggest that detection of c-jun mRNA could be an index of diagnosis of brain concussion and a sensitive marker of timing of injury after brain concussion.

关 键 词：法医病理学 脑震荡 C-JUN mRNA 原位杂交 损伤经过时间 

分 类 号：D919.4[医药卫生—法医学]