Establishment and characterization of primary lung cancer cell lines from Chinese population  

作　　者：Chao ZHENG Yi-hua SUN Xiao-lei YE Hai-quan CHEN Hong-bin JI 

机构地区：[1]Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]Department of Thoracic Surgery, Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China

出　　处：《Acta Pharmacologica Sinica》2011年第3期385-392,共8页中国药理学报（英文版）

基　　金：This work was supported by the National Basic Research Program of China （2010CB912102）, the National Natural Science Foundation of China （30871284 and 30971461）, the Chinese Academy of Sciences （KSCX1-YW-22）, and the Science and Technology Commission of Shanghai Municipality （08PJ14105, 09JC1416300）. Hong-bin JI is a scholar of the Hundred Talents Program of the Chinese Academy of Sciences.

摘　　要：Aim： To establish and characterize primary lung cancer cell lines from Chinese population. Methods： Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium （for non-small cell lung carcinomas （NSCLC）） or HITES medium （for small cell lung carcinomas （SCLC）） supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results： Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor （EGFR） kinase domain mutations. Conclusion： These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future stud- ies of the ethnic differences in lung cancer biology and drug response.Aim： To establish and characterize primary lung cancer cell lines from Chinese population. Methods： Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium （for non-small cell lung carcinomas （NSCLC）） or HITES medium （for small cell lung carcinomas （SCLC）） supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results： Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor （EGFR） kinase domain mutations. Conclusion： These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future stud- ies of the ethnic differences in lung cancer biology and drug response.

关 键 词：lung cancer primary cell lines epidermal growth factor receptor small cell lung carcinomas non-small cell lung carcinomas 

分 类 号：Q813[生物学—生物工程] S852.43[农业科学—基础兽医学]