Effects of sulfide on sulfate reducing bacteria in response to Cu(II), Hg(II) and Cr(VI) toxicity  

作　　者：SHENG YuXing CAO HongBin LI YuPing ZHANG Yi 

机构地区：[1]National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology,Beijing 100190,China [2]Key Laboratory of Green Process and Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Chinese Science Bulletin》2011年第9期862-868,共7页

基　　金：supported by the National Natural Science Foundation of China (20877075);the National High-Technology R&D Program of China (2006BAC02A05);the National Key Basic Research Program of China (2007CB613501)

摘　　要：Sulfate reducing bacteria (SRB) is identified as the primary organisms responsible for the treatment of heavy metal wastewater. However, most heavy metals can inhibit the growth of SRB during heavy metal treatment processes. Sulfide is a metabolic product of SRB and it can precipitate or reduce heavy metals. This study focused on the effects of sulfide on SRB resistance to Cu(II), Hg(I) and Cr(VI) toxicity. First, we considered the existence style of various heavy metals with and without sulfide addition by sequential extraction experiments. Second, the particle size distribution was evaluated and the cell structure during the metabolism of a SRB culture, containing different heavy metals, was analyzed by particle size distribution and TEM analyses. Third, the evolution of sulfate under the influence of different concentrations of heavy metals with and without sulfide addition was investigated to evaluate SRB activity. The results indicated that sulfide played an important role in alleviating and even eliminating the toxicity of Cu(II), Hg(II) and Cr(VI). We also discuss the mechanism of sulfide on SRB resistance to Cu(II), Hg(I) and Cr(VI) toxicity.Sulfate reducing bacteria (SRB) is identified as the primary organisms responsible for the treatment of heavy metal wastewater. However, most heavy metals can inhibit the growth of SRB during heavy metal treatment processes. Sulfide is a metabolic product of SRB and it can precipitate or reduce heavy metals. This study focused on the effects of sulfide on SRB resistance to Cu(Ⅱ), Hg(I) and Cr(VI) toxicity. First, we considered the existence style of various heavy metals with and without sulfide addition by sequential extraction experiments. Second, the particle size distribution was evaluated and the cell structure during the metabolism of a SRB culture, containing different heavy metals, was analyzed by particle size distribution and TEM analyses. Third, the evolution of sulfate under the influence of different concentrations of heavy metals with and without sulfide addition was investigated to evaluate SRB activity. The results indicated that sulfide played an important role in alleviating and even eliminating the toxicity of Cu(Ⅱ), Hg(Ⅱ) and Cr(VI). We also discuss the mechanism of sulfide on SRB resistance to Cu(Ⅱ), Hg(I) and Cr(VI) toxicity.

关 键 词：硫酸盐还原菌 CR(VI) 汞毒性 硫化铜 金属硫化物 铬(VI) 作者 废水生物处理 

分 类 号：X172[环境科学与工程—环境科学]