Direct Electrochemistry and Electrocatalysis of Hemoglobin Based on Nation-Room Temperature Ionic Liquids- Multiwalled Carbon Nanotubes Composite Film  

作　　者：张亚 郑建斌 

机构地区：[1]Department of Chemistry and Chemical Engineering, Yulin College, Yulin, Shaanxi 719000, China [2]Institute of Analytical Science, Northwest University, Xi'an, Shaanxi 710069, China

出　　处：《Chinese Journal of Chemistry》2011年第4期685-690,共6页中国化学（英文版）

基　　金：Project supported by the Natural Science Foundation of Shaanxi Province （No. 2010JM2017）, the Foundation of Shaanxi Province Education Department （No. 2010JK925） and the Foundation of Yulin College （No. 10GK02）.

摘　　要：A robust and effective composite film combined the benefits of Nation, room temperature ionic liquid （RTIL） and multi-wall carbon nanotubes （MWNTs） was prepared. Hemoglobin （Hb） was successfully immobilized on glassy carbon electrode surface by entrapping in the composite film. Direct electrochemistry and electrocatalysis of immobilized Hb were investigated in detail. A pair of well-defined and quasi-reversible redox peaks of Hb was obtained in 0.10 mol,L^-1 pH 7.0 phosphate buffer solution （PBS）, indicating that the Nafion-RTIL-MWNTs film showed an obvious promotion for the direct electron transfer between Hb and the underlying electrode. The immobilized Hb exhibited an excellent electrocatalytic activity towards the reduction of H2O2. The catalysis current was linear to H2O2 concentration in the range of 2.0× 10^-6 to 2.5× 10-4mol·L-1, with a detection limit of 8.0× 10-7 moloL-1 （S/N=3）. The apparent Michaelis-Menten constant （ Kapp ） was calculated to be 0.34 mmolo·L^-1 Moreover the modified electrode displayed a good stability and reproducibility. Based on the composite film, a third-generation reagentless biosensor could be constructed for the determination of H2O2.A robust and effective composite film combined the benefits of Nation, room temperature ionic liquid （RTIL） and multi-wall carbon nanotubes （MWNTs） was prepared. Hemoglobin （Hb） was successfully immobilized on glassy carbon electrode surface by entrapping in the composite film. Direct electrochemistry and electrocatalysis of immobilized Hb were investigated in detail. A pair of well-defined and quasi-reversible redox peaks of Hb was obtained in 0.10 mol,L^-1 pH 7.0 phosphate buffer solution （PBS）, indicating that the Nafion-RTIL-MWNTs film showed an obvious promotion for the direct electron transfer between Hb and the underlying electrode. The immobilized Hb exhibited an excellent electrocatalytic activity towards the reduction of H2O2. The catalysis current was linear to H2O2 concentration in the range of 2.0× 10^-6 to 2.5× 10-4mol·L-1, with a detection limit of 8.0× 10-7 moloL-1 （S/N=3）. The apparent Michaelis-Menten constant （ Kapp ） was calculated to be 0.34 mmolo·L^-1 Moreover the modified electrode displayed a good stability and reproducibility. Based on the composite film, a third-generation reagentless biosensor could be constructed for the determination of H2O2.

关 键 词：HEMOGLOBIN room temperature ionic liquid multi-walled carbon nanotube ELECTROCHEMISTRY ELECTROCATALYSIS 

分 类 号：O645.4[理学—物理化学] TB383[理学—化学]