重组水蛭素Ⅲ对半乳糖损伤的人晶状体上皮细胞热应激蛋白表达的影响  

Effects of Recombinant Hirudin Variant Ⅲ on the Expression of Heat Shock Proteins in Human Lens Epithelial Cells Damaged by Galactose

作　　者：欧瑜[1] 朱丹丹[1] 耿培[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2011年第2期115-118,共4页Pharmaceutical Biotechnology

基　　金：教育部科学技术研究重点项目(S092006)

摘　　要：探讨重组水蛭素Ⅲ(rHV3)对半乳糖损伤的人晶状体上皮细胞(hLEC)热应激蛋白(Hsp)表达的影响。采用125×10-3mol/LD-半乳糖建立人晶状体上皮细胞SAR01/04损伤模型,用重组水蛭素Ⅲ干预受损细胞,设置正常组、模型组、和重组水蛭素Ⅲ组,用倒置显微镜观察各组细胞密度和形态,RT-PCR检测人晶状体上皮细胞内3种热应激蛋白mRNA的表达。结果显示,125×10-3mol/LD-半乳糖导致人晶状体上皮细胞显著损伤,3种热应激蛋白,即Hsp70、Hsp27和αB-晶体蛋白的mRNA表达显著上调。重组水蛭素Ⅲ的干预使细胞形态趋于正常,细胞内Hsp70、Hsp27和αB晶体蛋白的mRNA表达显著下调。因此,重组水蛭素Ⅲ能有效缓解高浓度半乳糖导致的热应激蛋白应激耐受,从而保护晶状体上皮细胞。The aim of the paper is to investigate the effects of recombinant hirudin variant Ⅲ（rHV3） on the expression of heat shock proteins in human lens epithelial cell（hLEC） line SAR01/04 damaged by galactose.Damaged hLEC model was established using 125×10-3mol/L D-galactose,and rHV3 was used to interfere the damaged cells.There were three experimental groups： normal group,model group,and rHV3 group.The cells were observed under the phase-contrast microscope for any morphological changes.The expression of three heat shock proteins at the mRNA levels in cells was detected using RT-PCR.The cells were significantly damaged,and the mRNA levels of Hsp70、 Hsp27 and αB-crystallin were upregulated,under the condition of 125×10-3mol/L D-galactose.rHV3 prevented the cellular morphological changes and elevation of Hsp70、 Hsp27 and αB-crystallin mRNAs.These results indicated that rHV3 could attenuate stress tolerance of heat shock proteins insulted by D-galactose and have the positive function in protecting the hLEC.

关 键 词：人晶状体上皮细胞 重组水蛭素Ⅲ 半乳糖 热应激蛋白 

分 类 号：Q51[生物学—生物化学]