羊鲍野生群体遗传多样性与分化的研究  

Genetic diversity and differentiation of three wild populations of Haliotis ovina

作　　者：张桂玲[1] 黎中宝[1] 谢德华[1] 

机构地区：[1]集美大学水产学院,水产生物技术研究所,福建厦门361021

出　　处：《海洋科学》2011年第3期12-16,共5页Marine Sciences

基　　金：国家自然科学基金项目(30271013);国家留学回国人员科研启动基金[教外司留(2006)331号];福建省高等教育新世纪优秀人才支持计划资助项目[闽教科〔2006〕35];集美大学创新团队基金资助项目(2007A001)

摘　　要：运用随机扩增片段长度多态性DNA标记(RAPD)技术对中国海南省英州、安游和亚龙湾海域的3个羊鲍(Haliotis ovina)野生群体进行遗传多样性与分化的研究。利用14个10bp随机引物,在90个个体中共检测到72个位点,其中英州,安游和亚龙湾群体的多态位点数分别为65,61和62个,多态位点百分数(P)分别为90.28%、84.72%和86.11%;Shannon's信息指数(I)分别为0.443、0.456和0.400;Nei's基因多样性指数(H)分别为0.292、0.305和0.262。3个羊鲍群体间的遗传距离(D)介于0.0504～0.0731之间;群体间的遗传分化系数(GST)为0.0976。结果表明,羊鲍3个野生群体的遗传多样性水平较高,群体间的遗传分化较小。Random amplified polymorphic DNA（RAPD） technique was used to analyze three wild Haliotis ovina from Yingzhou,Yalongwan,and Anyou of Hainan Province.Seventy-two loci were found by 14 random primers,and Yingzhou,Yalongwan,Anyou populations having 65,61 and 62,respectively.The percentages of polymorphic loci（P） of the three populations were 90.28%,84.72%,and 86.11%,respectively;the Shannon＇s information index（I） was 0.443 in Yingzhou population,0.456 in Yalongwan population,and 0.400 in Anyou population;the Nei＇s gene diversity（H） were 0.292,0.305,and 0.262,respectively.Genetic distances（D）among the three populations were about 0.0504～0.0731.The genetic differentiation index GST among the three populations was 0.0976.Our study shows that the genetic diversity of H.ovina is at a high level,and the genetic differentiation is low.

关 键 词：羊鲍(Haliotis ovina) 遗传多样性 遗传分化 RAPD 

分 类 号：Q78[生物学—分子生物学] Q959.3