吸烟对慢性阻塞性肺疾病患者支气管肺泡灌洗液中T细胞功能的影响  

Clinical study on T lymphocytes influenced by smoking in bronchoalveolar lavage fluid of patients with chronic obstructive pulmonary disease

作　　者：陈菁[1] 徐清[1] 杨硕[1] 刘梦琳[1] 聂红[1] 孙洁民[1] 

机构地区：[1]武汉市第一医院呼吸科,430022

出　　处：《实用医学杂志》2011年第9期1565-1567,共3页The Journal of Practical Medicine

基　　金：湖北省自然科学基金资助项目(编号:2005ABA210)

摘　　要：目的:评价吸烟对慢性阻塞性肺疾病(COPD)患者急性期、稳定期支气管肺泡灌洗液(BALF)中T细胞功能的影响。方法:根据患者吸烟史将患者分为无吸烟史的正常组(A组)、非吸烟的COPD组(B组)、持续吸烟的COPD组(C组)、已戒烟的COPD组(D组);用流式细胞术检测各组患者急性期、稳定期BALF中CD3+、CD4+、CD8+T细胞和CD+4/CD+8水平。结果:COPD患者无论在急性期还是稳定期,BALF中CD+3、CD+4、CD+8、CD+4/CD+8与正常组比较均有明显差异(P<0.05);持续吸烟COPD患者与非吸烟COPD患者、已戒烟的COPD患者相比较,急性期CD+4、CD+4/CD+8显著下降(P<0.05)、CD+8显著上升(P<0.05);稳定期CD+3、CD+4、CD+8无明显差异(P>0.05),CD+4/CD+8明显下降(P<0.05)。结论:持续吸烟的COPD患者在急性期和稳定期气道T细胞功能明显低下。Objective To investigate influence on T lymphocytes influenced by smoking in bronchoalveolar lavage fluid （BALF） of patients with chronic obstructive pulmonary disease （COPD） during the acute and stable periods. Methods According to the smoking history, the patients were divided into 4 groups： Normal group without smoking history （Group A）, COPD group without smoking history （Group B）, COPD group with continuous smoking history （Group C）, COPD group with quitting smoking at least 10 years （Group D）. Flow cytometry was used to test cells of CD3＋, CD4＋, CD8＋ T cells and the levels of CD4＋/CD8＋ in BALF in acute and stable period respectively. Results Compared with Group A, the levels of CD3＋, CD4＋, CD8＋, CD4＋/ CD8＋ in BALF were significantly different in the COPD groups during their acute or stable periods （P 〈 0.05）. Compared with Group B and Group D, CD4＋ and CD4＋/CD8＋ were declined （P 〈 0.05） as well as CD8＋ was raised （P 〈 0.05） in Group C during the acute periods; yet, there was no difference in CD3＋,CD4＋,CD8＋ （P 〉 0.05） while CD4＋/CD8＋ was dropped obviously （P 〈 0.05） during the stable periods. Conclusions The immunological function of T lymphocyte in the airway was dropped significantly in patients with COPE） during acute and stable periods who smoked consistently.

关 键 词：肺疾病 慢性阻塞性 吸烟 支气管肺泡灌洗液 T细胞 

分 类 号：R563.9[医药卫生—呼吸系统]