七叶皂苷钠对小鼠肝癌H22移植瘤的抗肿瘤作用机制  

Antitumor effect of sodium aescinate in mice with H22 hepatoma and the mechanism

作　　者：聂昕[1] 郭维[1] 徐波[1] 崔景荣[1] 

机构地区：[1]北京大学天然药物及仿生药物国家重点实验室,北京100083

出　　处：《中国新药杂志》2011年第8期688-691,共4页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-010)

摘　　要：目的:通过体内模型研探讨七叶皂苷钠对H22小鼠肝癌移植瘤的抗肿瘤作用机制。方法:采用ICR小鼠H22荷瘤肝癌模型观察七叶皂苷钠抗肿瘤作用,采用Western Blot分析肿瘤组织中相关蛋白的表达变化,通过免疫组化分析肿瘤组织中CD31变化,评价微血管密度。结果:1.4和2.8 mg.kg-1七叶皂苷钠对H22的抑瘤率分别为19.2%,40.7%。免疫组化结果显示,七叶皂苷钠能够显著降低肿瘤内部微血管密度,低高剂量组可分别达到44.1%和48.5%。Western Blot证实,七叶皂苷钠能不同程度下调cyclinD1、cdk2、cyclinE、VEGF、p-Akt等蛋白的表达,抑制p65的核转位。结论:七叶皂苷钠有一定的抗肿瘤作用,其机制可能与阻滞细胞周期,抑制血管新生以及干扰信号转导有关。Objective：To investigate the antitumor effect of sodium aescinate in vivo and the mechanisms.Methods： A mouse model of H22 hepatoma was employed to investigate the antitumor effect of sodium aescinate.Samples from tumor tissue were analyzed for the tumor growth associated proteins through Western Blot.Immunohistochemistry was performed to evaluate the microvessel density through CD31 labeling.Results： Sodium aescinate inhibited tumor growth and reduced tumor weight by 19.2% and 40.7% at doses of 1.4 and 2.8 mg·kg-1,respectively.Immunohistochemical analysis revealed that sodium aesciante in low/high doses reduced microvessel density by 44.1% and 48.5% compared with control group.Western Blot results showed that sodium aescinate downregulated the expression of cyclin D1,cdk2,cyclinE,VEGF and p-Akt.In addition,sodium aescinate also blocked the nuclear translocation of p65.Conclusion： Sodium aescinate has a tumor-inhibiting effect in vivo.It can regulate cell cycle,angiogenesis and signal transduction,which might contribute to its antitumor effect.

关 键 词：七叶皂苷钠 细胞周期相关蛋白 微血管密度 NFΚ-B 血管内皮生长因子 

分 类 号：R979.1[医药卫生—药品]