过氧化氢-2,4-二氯苯酚-4-氨基安替比林体系催化光度法测定血红细胞中的铁  

Catalytic Spectrophotometric Determination of Trace Iron with Hydrogen Peroxide-2,4-Dichlorophenol-4-Amino Antipyrine System

作　　者：唐波[1] 刘阳[1] 王洪鉴[1] 孙天麟[1] 

机构地区：[1]山东师范大学化学系,济南250014

出　　处：《分析化学》1999年第12期1397-1400,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.29975016);山东省杰出青年科学家基金(No.98015);山东省自然科学基金(No.97B13036)

摘　　要：基于在十二烷基硫酸钠存在下的弱碱性介质中Fe(Ⅲ)对H_2O_2氧化2,4-二氯苯酚(2,4-DCP)与4-氨基安替比林(4-AAP)偶联显色反应的催化作用,建立了检出限为7.90μg/L.线性范围为0～1.25mg/L的新催化光度分析法.方法用于血红细胞中铁的测定,相对标准偏差与回收率分别为2.6%与98.8%～101%,结果满意.The catalytic coupling reaction of 2,4-dichlorophenol and 4-amino antipyrine with hydrogenperoxide by iron(Ⅲ) in a weak base medium was studied and its optimum conditions were established .The absorbance of the red product was measured at 502 nm with a detection hrnit of 7.9μg/L. The linearrange of this method was 0～1.25 mg/L and the relative standard deviation was 2.6%. The proposedmethod was used to determine trace iron in erythrocyte with satisfactory results.

关 键 词：铁 分光光度法 催化动力学 偶联反应 血红细胞 

分 类 号：Q26[生物学—细胞生物学] O657.32[理学—分析化学]