RP-HPLC法测定护肤品中白藜芦醇的含量  

Determination of Resveratrol in Cosmetics by High Performance Liquid Chromatography

作　　者：史先敏[1,2] 严泽民[2] 何宏轩[2] 段明星[1] 

机构地区：[1]生物膜与膜生物工程国家重点实验室清华大学生命科学学院,北京100084 [2]江苏隆力奇生物科技股份有限公司研发中心,常熟215555

出　　处：《香料香精化妆品》2011年第2期38-39,51,共3页Flavour Fragrance Cosmetics

摘　　要：建立RP-HPLC测定化妆品中白藜芦醇含量的方法。色谱柱为Shimadzu ODS C18柱(4.6mm×150mm,5m),流动相为甲醇:0.05%磷酸水溶液(50:50),等度洗脱,检测波长306nm,流速1mL/min,上样量10μL,柱温为室温。结果表明白藜芦醇在0～200mg/L呈良好的线性关系,相关系数为0.9997,护肤品样品平均加标回收率为98.8%,相对标准偏差为2.06%。本方法操作简便,结果准确可靠,适于化妆品中白藜芦醇的含量测定。An RP HPLC method for determination of resveratrol in cosmetics was reported. Separation was achieved on a Shimadzu ODS Cl8 column （4.6 mm×150mm,5 m） with methanol/0.05% phosphoric acid （50/50, V/V）. The detection wavelength is 306 nm and the flow rate is 1.0 mL/min. It was found that resveratrol had good linear relationship in the range of 0 - 200 mg/L and the correlation coefficients of linear calibration curves was 0. 999 7. The average recovery of resveratrol is 98.8% and the relative standard deviation is 2.06%.

关 键 词：白藜芦醇 RP-HPLC 化妆品 

分 类 号：R194.7[医药卫生—卫生事业管理]