山西汉族17个Y-STR基因座遗传多态性及遗传关系  

Polymorphism of 17 Y-STR loci in Shanxi Han population and genetic relationship with 13 populations

作　　者：石美森[1] 百茹峰[1] 傅博[1] 

机构地区：[1]中国政法大学证据科学教育部重点实验室,北京100088

出　　处：《遗传》2011年第3期228-238,共11页Hereditas（Beijing)

基　　金：国家自然科学基金青年项目(编号:30801320);教育部科学技术研究重点项目(编号:109037)和教育部新世纪优秀人才支持计划(编号:NCET-10-0773)资助

摘　　要：为了调查山西汉族群体17个Y-STR基因座的多态性分布,探讨其群体遗传学及法医学应用价值,文章应用Y-filer TM试剂盒检测222名山西汉族无关男性个体的17个Y-STR基因座,用ABI3130遗传分析仪进行基因分型,计算等位基因频率及单倍型多样性,并结合已公开发表的国内其他13个群体相关数据资料,分析山西汉族群体遗传距离和聚类关系。结果：山西汉族个体中共检出219种单倍型,单倍型多样性为0.9999;基因多样性GD值在0.3894（DYS391）~0.9755（DYS385a/b）。从遗传距离分析发现,山西汉族与吉黑汉族之间的遗传距离最近（？0.0001）,与台湾群体（0.0152）之间的遗传距离相对较远。结果表明该17个Y-STR基因座在山西汉族群体中具有丰富的遗传多态性,对建立Y染色体STR数据库、研究群体遗传学和进行法医学应用有重要意义。To investigate the genetic polymorphisms of 17 Y-chromosomal short tandem repeats（Y-STR） loci in Shanxi Han population and to explore their forensic application values and genetic relationship with neighboring populations,17 Y-STR in 222 unrelated healthy Shanxi Han individuals were amplified with Y-filerTM System,and the PCR products were detected with 3130 Genetic Aanalyzer.The allele frequencies and haplotype diversity were calculated.Y-STR data of an-other 13 populations were collected from publications.Cluster analysis and phylogenic trees were applied to show the ge-netic distance among the populations.As a result,a total of 219 haplotypes were identified,and the observed haplotypes diversity value was 0.9999.The gene diversity values（GD） for each locus ranged from 0.3894（DYS391） to 0.9755（DYS385a/b）.Comparing with 13 populations,the genetic distance between Shanxi and Jihei Han populations was the smallest（？0.0001）,while the genetic distance between Shanxi and Taiwan population was the largest（0.0152）.The phy-logenetic tree was similar to the results of clustering analysis.Multiplex detection of the 17 Y-STR loci revealed a highly polymorphic genetic distribution,which would be very powerful for establishing a Y-STR database,for population genetics and forensic practice.

关 键 词：Y染色体 短串联重复序列 单倍型 遗传多态性 遗传距离 

分 类 号：Q987[生物学—遗传学]