氧化低密度脂蛋白对人脐静脉内皮细胞connexin43表达的影响  

Effect of oxidized LDL on expression of connexin43 in cultured human umbilical vein endothelial cells

作　　者：章顺荣[1] 洪鸣鸣[1] 高越[1] 王晓楠[1] 封菲[1] 

机构地区：[1]杭州市第一人民医院老年医学科,浙江杭州310006

出　　处：《中国病理生理杂志》2011年第3期589-592,共4页Chinese Journal of Pathophysiology

基　　金：浙江省医药卫生科技计划资助项目(No.2008B145)

摘　　要：目的:研究氧化低密度脂蛋白(ox-LDL)对体外培养的人脐静脉内皮细胞缝隙连接蛋白connex-in43表达的影响。方法:体外培养的内皮细胞分为4组:对照组,50 mg/L、100 mg/L、200 mg/L ox-LDL干预组,干预24 h后通过RT-PCR方法检测各组connexin43 mRNA的表达有无差别;通过免疫细胞化学方法检测对照组和100 mg/L ox-LDL干预组之间connexin43蛋白的表达水平有无差别。结果:不同浓度(50 mg/L、100 mg/L、200mg/L)的ox-LDL干预24 h能引起内皮细胞connexin43 mRNA的表达增加(P<0.01);100 mg/L的ox-LDL干预24 h内皮细胞connexin43蛋白表达增加(P<0.01)。结论:Ox-LDL短期干预可引起人脐静脉内皮细胞connex-in43 mRNA和蛋白表达水平增加。AIM：To investigate the effect of oxidized LDL （ox-LDL） on the expression of gap junction protein connexin43 in cultured human umbilical vein endothelial cells （HUVECs） in vitro.METHODS：Human umbilical vein endothelial cells cultured in normal condition were divided into blank control group,50 mg/L,100 mg/L and 200 mg/L ox-LDL intervention groups.The mRNA expression of connexin43 in cultured HUVECs was detected with RT-PCR method;while the protein level of connexin43 was determined by the method of immunocytochemistry in the control and 100 mg/L ox-LDL intervention groups 24 h after ox-LDL was given.RESULTS：Different concentrations （50 mg/L,100 mg/L,200 mg/L） of ox-LDL up-regulated mRNA expression of connexin43 in cultured HUVECs after 24 h intervention （P〈0.01）.The protein level of connexin43 in cultured HUVECs intervened with 100 mg/L Ox-LDL for 24 h was up regulated as compared to the control cells （P〈0.01）.CONCLUSION：Ox-LDL may up-regulate the expression of connexin43 at mRNA and protein levels in cultured human umbilical vein endothelial cells within short time,indicating that connexin43 plays an important role in the pro-atherosclerotic effect of Ox-LDL.

关 键 词：连接蛋白43 脂蛋白类 低密度 人脐静脉内皮细胞 

分 类 号：R541.4[医药卫生—心血管疾病]