黄瓜霜霉病抗性相关基因的初步研究  

Preliminary Study on Resistance-related Genes in Cucumber Inoculated with Pseudoperonospora cubensis

作　　者：李建吾[1] 司胜伟[1] 胡建斌[1] 刘丽君[1] 

机构地区：[1]河南省高等学校园艺植物生理与遗传改良重点学科开放实验室,河南省果树瓜类生物学重点实验室 河南农业大学园艺学院,郑州450002

出　　处：《园艺学报》2011年第3期471-478,共8页Acta Horticulturae Sinica

基　　金：‘十一五’国家科技支撑计划项目(2009BADB8B02);河南省科技攻关重点项目(102102110037);国家高技术研究发展计划(863计划)项目(2007AA10Z177)

摘　　要：以黄瓜高抗霜霉病自交系为材料,构建了两个抑制差减文库,经反向Northern杂交筛选、测序和序列比对,筛选到3个未知功能抗病相关基因。荧光实时定量RT-PCR分析结果表明,未知功能抗病相关基因2I15(GD254229)、3C19(GD254243)和1O08(GD254207)在抗病自交系接种霜霉病病原菌后,可早期高丰度特异上调表达,而在感病自交系中特异上调表达丰度较低或被特异抑制表达。水杨酸(SA)可诱导1O08特异上调表达,抑制2I15和3C19的表达;茉莉酸(JA)处理可诱导1O08特异上调表达,而抑制2I15的表达。ABA处理可显著诱导3C19和1O08特异上调表达,而抑制2I15的表达。机械伤害、高盐、冷害和热激等其它非生物胁迫可显著抑制或诱导这些基因的表达,因此推测,所筛选到的功能未知基因可能具有参与生物和非生物胁迫反应的作用。In order to identify the genes associated with cucumber（Cucumis sativus L.）resistance against Pseudoperonospora cubensis（Berk. and Curk.）Rostov.,two cDNA libraries were constructed by suppression subtractive hybridization（SSH）. After differential screening by reverse northern dot blot hybridization,sequencing and BLAST,2I15（GD254229）,3C19（GD254243）and 1O08（GD254207）differential expressed genes with unknown function were isolated,furthermore,expression patterns of the genes were monitored by real-time quantitative RT-PCR. The results indicated that these genes were differentially up-regulated earlier and more quantitative in the resistant inbred line than the susceptible inbred line. Moreover,1O08 and 2I15 could be differentially up- or down- regulated,respectively,by salicylic acid（SA）,jasmonic acid（JA）and abscissic acid（ABA）,and 3C19 could be induced or suppressed significantly by ABA and SA,respectively. Mechanical wounding,NaCl stresses,cold and heat shock could induce or suppress these genes differential expression,suggesting that these genes may have some functions involved in biotic and abiotic stresses.

关 键 词：黄瓜 霜霉病 抑制差减杂交 抗性相关基因 

分 类 号：S642.2[农业科学—蔬菜学]