南方根结线虫辣椒Me3毒性群体适合度代价及专化性分析  

Fitness Cost and Specialization of Pepper Me3 Virulent Population in the Parthenogenetic Nematode Meloidogyne incognita

作　　者：蒋丽芬[1] 茆振川[1] 陈国华[1] 杨宇红[1] 谢丙炎[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所国家蔬菜遗传改良重点开放实验室,北京100081

出　　处：《园艺学报》2011年第3期479-486,共8页Acta Horticulturae Sinica

基　　金：国家重点基础发展计划项目(2009CB119000);现代农业产业技术体系建设专项;公益性行业(农业)科研专项(nyhyzx07-050);‘十一五’国家科技支撑计划项目(2006BAD08A08);国家自然科学基金项目(30971905);国家大宗蔬菜产业技术体系项目

摘　　要：将南方根结线虫自然群体在含有Me3抗线虫基因的辣椒HDA149上连续选择15代,获得了一对针对辣椒(Capsicum annuum L.)抗线虫基因Me3的南方根结线虫毒性和无毒近等基因系。通过将Me3毒性线虫接种在抗病辣椒HDA149、感病辣椒茄门以研究适合度代价,结果显示,南方根结线虫Me3毒性群体在感病辣椒上存在适合度代价,适应值仅为76%。将Me3毒性线虫接种在含有其它抗线虫基因(Mi、Me)的番茄和辣椒上研究南方根结线虫Me3毒性的专化性,结果表明Me3毒性线虫不能在含有Mi抗线虫基因的番茄上产生根结和卵块,但是可以在含有其它Me基因的辣椒上产生卵块。说明南方根结线虫Me3毒性对抗性基因Me存在专化性。A natural population of Meloidogyne incognita from field was inoculated on resistant pepper cultivar HDA149,which carried a single dominant nematode-resistant gene Me3. For 15 successive generations,a pair of near-isogenic lines of virulent and avirulent nematode to Me3 was obtained. The fitness cost was analyzed with the reproductive fitness（RF）,that Me3 virulent nematodes on the resistant pepper cultivar HDA149 and susceptible pepper cultivar Qiemen. The result indicated that the fitness cost was associated with virulence on susceptible host Qiemen and the fitness value was only 76%. The specialization was detected by the reproductive index of Me3 virulent nematodes on pepper and tomato which contained different resistant genes（Mi,Me）. The Me3 virulent nematodes didn’t produce any root-knots or egg-masses on resistant tomato which with Mi gene,but the virulent nematodes produced egg-masses on resistant pepper which carried other Me genes,it showed that the Me3 virulence had specialization.

关 键 词：南方根结线虫 辣椒 Me3基因 毒性群体 适合度代价 专化性 

分 类 号：S641.3[农业科学—蔬菜学]