芍药野生居群父系分析与遗传结构研究  

Paternity Identification and Genetic Structure Analysis on the Wild Population in Paeonia lactiflora Pallas (Paeoniaceae)

作　　者：胡文清[1,2] 鲁衡[1] 刘伟 袁俊霞[1] 张大明[1] 

机构地区：[1]中国科学院植物研究所系统与进化国家重点实验室,北京100093 [2]中国科学院研究生院,北京100049

出　　处：《园艺学报》2011年第3期503-511,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(39870055);国家重点基础研究发展计划项目(2009CB119102)

摘　　要：采用AFLP分子标记,对中国芍药(Paeonia lactiflora Pallas)代表居群——山西省交城县嵛岭山居群半同胞家系进行了父本组成、繁殖适合度、花粉散布、代际间遗传多样性水平和空间遗传结构的分析。9对引物共得到清晰判读的AFLP扩增带255条,其中多态性条带113条。对半同胞家系的父系分析结果表明,25个候选父系亲本在95%的可信度内检测出总的雄性繁殖贡献率为78.07%。芍药仅存在微弱的自交(自交率为2.25%),花粉散布以5m为中心呈现尖峰分布,最远可达50m,平均花粉散布距离23m。亲代的多态性比率(P)为42.75%,平均Nei's基因多样度指数(He)为0.1683±0.2083,Shannon指数(I)为0.2462±0.2982;子代P为52.94%,He为0.1921±0.2055,I为0.2855±0.2920。0～32m距离范围内呈现显著的空间遗传结构。研究结果表明,芍药作为一种异交植物,花粉散布尺度小,受地理距离的影响显著,花粉散布符合邻近距离散布模式;遗传多样性水平不高,亲本和子代代际之间的遗传多样性水平差别不明显;近距离范围内存在显著的空间遗传结构。Paternal identification and constitution,reproductive fitness,pollen dispersal,genetic diversity levels and genetic distance in the semi-sibling families of a wild population of Paeonia lactiflora （Paeoniaceae）,were analyzed based on AFLP markers. The pedigree between parental and seed populations was reconstructed,and gene flow,including its obstacles among pollen donors and acceptors within the population,was revealed. Nine pairs of selective AFLP primers amplified 255 clear bands,among which 113 were polymorphic. Analysis on the paternity of the semi-sibling families showed that selfing was as low as 2.25%,and total reproductive fitness with 95% confidence interval was 78.07%. The pollen dispersal showed a leptokurtic distances around a median of 5 meters（average 23 meters）,reaching a farthest distance of 50 meters. The polymorphic ratio of parental population was 42.75%,the genetic diversity index,He,was 0.1683 ± 0.2083,and I was 0.2462 ± 0.2982;whereas P in seed population was 52.94%,He was 0.1921 ± 0.2055,and I was 0.2855 ± 0.2920. The range of genetic distances between the seeds（2.646–7.937）was slightly wider than that between the parental individuals（3.742–8.062）. The spatial genetic structure was visible only from 0 to 32 meters. The result showed that P. lactiflora is a strict out breeding species,but the distance of pollen dispersal was short,being influenced significantly by the geographic distances,which was accordance to neighbor dispersal model. The genetic diversity of the wild population was very low,and the genetic diversity level of the parental individuals was similar to that of the seeds. Merely in short distance the genetic structure was significant.

关 键 词：芍药 父本分析 花粉散布 遗传结构 

分 类 号：S682.12[农业科学—观赏园艺]