COX-2、CDKN2A在慢性乙型肝炎、肝硬化肝组织中的表达及意义  

The study of the expression of COX-2 and CDKN2A in chronic hepatitis B and liver cirrhosis

作　　者：高保华[1] 刘冰[2] 郑建云[2] 吴方雄 汪雯[1] 李雷[1] 牛春燕[1] 

机构地区：[1]西安医学院附属医院消化内科,西安710077 [2]西安医学院附属医院病理科,西安710077

出　　处：《临床肝胆病杂志》2011年第3期273-276,共4页Journal of Clinical Hepatology

摘　　要：目的了解慢性乙型肝炎(CHB)、肝硬化肝组织中COX-2、CDKN2A的表达及其与肝脏损伤程度的关系。方法经免疫学及肝穿刺病理学证实为CHB患者46例、乙型肝炎肝硬化17例;正常肝组织10例(来自肝脏良性肿瘤手术切除的标本)。应用病理学判断肝组织的炎症及纤维化程度。采用免疫组化方法、半定量逆转录聚合酶链反应(RT-PCR)和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。结果 (1)CDKN2A mRNA第一、第二外显子在正常肝、CHB及肝硬化组织的表达水平及CDKN2A蛋白的半定量分析差异有统计学意义(F=28.75、24.86、26.43,P=0.036、0.041、0.038)。(2)正常肝、CHB及肝硬化组织中的COX-2、CDKN2A蛋白水平的表达差异有统计学意义(χ2=13.48、15.73,P=0.042、0.021);肝组织炎症不同级别的COX-2、CDKN2A蛋白水平的表达差异具有统计学意义(χ2=22.45、23.67,P=0.038、0.027);肝组织纤维化不同程度的COX-2、CDKN2A蛋白水平的表达差异具有统计学意义(χ2=24.16、22.53,P=0.024、0.037)。结论 COX-2、CDKN2A表达与CHB、肝硬化发生发展过程明显相关,COX-2、CDKN2A可作为反应HBV相关肝病进展程度的良好指标。Objective To investigate the expression of cyclooxygenase-2（COX-2） and CDKN2A in chronic hepatitis B and liver cirrhosis tissues and the relationship between the expression and the liver injury.Methods There were 46 patients with chronic hepatitis B and 17 cases with liver cirrhosis were confirmed through the immunology of liver biopsy;There were 10 normal liver tissues from benign tumors of the liver specimens.Liver inflammation and fibrosis was determined by Pathology.The expression of COX-2 and CDKN2A was determined by immunohisto-chemistry,semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and Western blot.Results（1） The expression level of the first and second exon of CDKN2A mRNA and the semiquantitative analysis of CDKN2A protein in the normal liver、hepatitis B and cirrhosis were significant（F=28.75,24.86,26.43,P=0.036,0.041,0.038）.（2） The protein level＇s expression of COX-2,CDKN2A in normal liver,hepatitis B,cirrhosis was significantly different（χ2=13.48,15.73,P= 0.042,0.021）;The protein level＇s expression of COX-2,CDKN2A in liver inflammation with different levels was significantly different（χ2=22.45,23.67,P=0.038,0.027）;The protein level＇s expression of COX-2,CDKN2A in Liver fibrosis with different degrees was significantly different（χ2=24.16,22.53,P=0.024,0.037）.Conclusion The expression of COX-2 and CDKN2A was related to the development of process of hepatitis and cirrhosis.The COX-2 and CDKN2A may be used as a good indicator of HBV-related liver disease progress.

关 键 词：环氧化酶2 周期素依赖激酶抑制剂p16 肝炎 乙型 慢性 肝硬化 基因表达 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]