肾移植受者外周血中CD4^+ CD25^+ FoxP3^+调节性T细胞比例在评价其免疫状态中的作用  

Role of CD4^+ CD25^+ FoxP3^+ Treg proportion in renal transplant recipients in evaluation of immune tolerance:a trans-vivo delayed type hypersensitivity assay

作　　者：韩容[1] 何跃[1] 刘宏[1] 吴雄飞[1] 

机构地区：[1]第三军医大学西南医院肾科,重庆400038

出　　处：《第三军医大学学报》2011年第7期738-740,共3页Journal of Third Military Medical University

基　　金：第三军医大学西南医院临床创新基金(SWH2006A003)~~

摘　　要：目的结合转移体内迟发超敏反应检测法初步探讨外周血中CD4+CD25+FoxP3+Treg的比例对亲体肾移植受者免疫状态评估的作用。方法收集西南医院肾科门诊随访的2005-2008年间手术的亲体肾移植供者和受者各30例,进行转移体内迟发超敏反应检测(trans-vivodelayed type hypersensitivity-assay,Transvivo-DTH)实验,根据实验结果将患者分为耐受组和排斥组;运用流式细胞技术检测2组中受者外周血单个核细胞(PBMCs)中CD4+CD25+FoxP3+Treg比例。结果耐受组和排斥组裸鼠掌肿胀(同时注入受者PBMCs和供者抗原的脚掌)厚度分别为(1.72±0.44)×10-2、(8.05±2.26)×10-2mm,前者明显高于后者(P<0.05),2组受者外周血中CD4+CD25+FoxP3+Treg的比例分别为(9.23±1.79)%和(1.03±1.04)%,耐受组明显高于排斥组(P<0.05)。耐受组和排斥组裸鼠脚掌皮下炎性水肿的厚度均与外周血中CD4+CD25+FoxP3+Treg的比例呈负相关(P<0.05,r分别为0.82和0.87)。结论受者外周血中CD4+CD25+FoxP3+Treg的比例能够反应受者T细胞对供者抗原发生免疫反应的程度。外周血中CD4+CD25+FoxP3+Treg的比例在发现移植受者是否已有耐受方面有一定的参考价值。Objective To investigate the role of the proportion of CD4＋CD25＋FoxP3＋Treg in peripheral blood in evaluating immune state of pro-kidney transplant recipients with the trans-vivo delayed type hypersensitivity（trans vivo-DTH） assay.Methods A total of 30 donors and their corresponding recipients who received living related kidney transplantation during 2005 to 2008 in Southwest Hospital were randomly selected.All these recipients had sound transplantation and regular of follow-up.Trans vivo-DTH assay was used to divide the patients into tolerance group and rejection group.After the recipients＇ PBMCs and donors＇ antigen were injected into the footpads immunodeficient mice,the swelling was measured to evaluate the cellular immune response of the recipients to donors.The proportion of CD4＋CD25＋FoxP3＋Treg in the peripheral blood of the recipients was detected by flow cytometry.Results According to the results of trans vivo-DTH assay,there were 23 patients in tolerance group and 7 patients in rejection group.The inflammatory swelling of the footpads were（1.72±0.44）×10-2 and（8.05±2.26）×10-2 mm respectively in the mice from tolerance group and rejection group,with the former significantly lower than the later（P0.05）.The proportion of CD4＋CD25＋FoxP3＋Treg were（9.23±1.79）% and（1.03±1.04）% respectively in the tolerance group and rejection group.Significant difference was found in the proportion of CD4＋CD25＋FoxP3＋Treg（P0.05）.The inflammatory swelling of mouse footpads of the tolerance group and rejection group was negative correlated with the proportion of CD4＋CD25＋FoxP3＋Treg in peripheral blood（P0.05,r=0.82 and 0.87）.Conclusion The proportion of CD4＋CD25＋FoxP3＋Treg in recipients＇ peripheral blood can reflect the immunoreactions of recipient T cells to donors＇ antigen.The proportion of CD4＋CD25＋FoxP3＋Treg in peripheral blood can be used as a reference to indicate tolerance after renal transplantation.

关 键 词：转移体内迟发过敏反应检测法 TREG 移植 排斥反应 免疫 

分 类 号：R392.4[医药卫生—免疫学] R692[医药卫生—基础医学]