干细胞微环境的体外模拟  

Simulation of stem cell microenvironment in vitro

作　　者：邱云[1,2,3] 郑青[1,2,3] 萧树东[1,2,3] 汪铮[1,2,3] 

机构地区：[1]上海交通大学医学院附属仁济医院消化科 [2]上海市消化疾病研究所 [3]上海交通大学MED-X仁济干细胞临床研究中心,上海市200001

出　　处：《中国组织工程研究与临床康复》2011年第6期1123-1126,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30971468)~~

摘　　要：背景:细胞外基质是组织微环境的重要组分,为维持细胞功能提供了关键的生化和物理信号。目的:对体外模拟骨髓干细胞微环境的研究进展进行综述,着重于介绍体外再造细胞外基质。方法:应用计算机检索Pubmed(1999-01/2010-10)、Embase(1999-01/2010-10)数据库中关于干细胞微环境的文章,以"mesenchymal stem cells;extracellular matrix;niche;growth factor modulation;culture in vitro"为检索词进行检索。初检得到321篇文献,根据纳入标准选择关于干细胞微环境的23篇文献进行综述。结果与结论:骨髓源性的细胞外基质培养体系能保持间充质干细胞的特性,对纯化这些细胞和形成更多数量的同质间充质干细胞提供了便利。使用3D合成材料建立一个独特的骨架,包被组织特异性的间充质干细胞蛋白,并添加正确的生长因子组合,有利于调控间充质干细胞的分化方向。BACKGROUND：Extracellular matrix（ECM） is an important component of the cellular niche in a tissue,supplying critical biochemical and physical signals to sustain cellular functions.OBJECTIVE：To review the research progress of in vitro simulation of bone marrow stem cell microenvironment,and to introduce reconstruction of ECM in vitro.METHODS：The database of Pubmed（1999-01/2010-10） and Embase（1999-01/2010-10） was utilized to search the related articles about stem cell microenvironment.Key words were ＂mesenchymal stem cells,extracellular matrix,niche,growth factor modulation,culture in vitro＂.There were 321 articles after the initial survey.A total of 23 articles related to stem cell microenvironment were selected according to inclusion criteria. RESULTS AND CONCLUSION：The established bone marrow-derived ECM culture system retains mesenchymal stem cell properties and may facilitate efforts to purify these cells and to produce a large number of relatively homogenous mesenchymal stem cells.Establishment of a unique scaffold built by 3D synthetic materials coated with tissue-specific ECM proteins and deposited with an appropriate combination of growth factors will facilitate the control of the differentiation direction of mesenchymal stem cells.

关 键 词：微环境 间充质干细胞 细胞外基质 生长因子调控 体外培养 

分 类 号：R318[医药卫生—生物医学工程]