兔耳瘢痕成熟过程中血管内皮细胞生长因子与缺氧诱导因子1α的表达  

Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during scar mutation in rabbit ears

作　　者：张刚[1] 谭军[2] 李高峰[2] 

机构地区：[1]浏阳市人民医院烧伤整形科,湖南省浏阳市410300 [2]湖南省人民医院整形及激光美容科,湖南省长沙市410005

出　　处：《中国组织工程研究与临床康复》2011年第7期1191-1194,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:尽管近20年来在组织、细胞和分子各个水平上展开了瘢痕的研究,但瘢痕形成的完整机制尚未阐明,探索瘢痕的形成的分子机制,寻求理想的瘢痕防治方法是医学研究和实践中一项紧迫而艰巨的任务。目的:观察兔耳瘢痕从形成到消退成熟过程中血管内皮细胞生长因子与缺氧诱导因子1α变化,探讨瘢痕形成的分子机制。方法:制作兔耳瘢痕从形成到消退成熟的动物模型,收集兔耳术后14,30,60和90d瘢痕和正常皮肤,应用苏木精-伊红染色方法观察兔耳术后各时间点瘢痕的组织形态学变化,免疫组织化学法方法检测术后各时间点血管内皮细胞生长因子、缺氧诱导因子1α在瘢痕组织中的表达。结果与结论:术后14d瘢痕组织中血管内皮细胞生长因子和缺氧诱导因子1α表达较其他时间点和正常组织均升高,而随着时间的延长,瘢痕的萎缩成熟,两者的表达水平逐渐降低,并在术后90d达到正常组织表达水平。结果表明缺氧诱导因子1α与血管内皮细胞生长因子在瘢痕组织内氧分压的变化过程中起到一定的调节作用。BACKGROUND：Scar studies have cover histological,cellular and molecular levels nearly 20 years,but the mechanism of scar formation is not clarified.How to explore an ideal method for preventing and treating scar is an urgent and difficult task in both medical field and clinical application.OBJECTIVE：To investigate molecular mechanism of scar formation by observing expression of vascular endothelial growth factor（VEGF） and hypoxia-inducible factor-1 α（HIF-1α） during scar mutation in rabbit ears.METHODS：A rabbit ears scar animal model was prepared,normal skin and scars at 14,30,60 and 90 days were collected.Histomorphological changes of scars were observed by hematoxylin-eosin staining and the expression of VEGF and HIF-1α was detected by immunohistochemical method.RESULTS AND CONCLUSION：Compared with other time points,the expression of VEGF and HIF-1α was greater at 14 days after operation,and gradually decreased with time prolonged and scar mature,which close to normal levels at 90 days after operation.The findings demonstrated that HIF-1α and VEGF play a regulation role in oxygen partial pressure changes of scar tissues.

关 键 词：瘢痕 血管内皮细胞生长因子 缺氧诱导因子1Α 动物模型 兔 

分 类 号：R318[医药卫生—生物医学工程]