通瘀化痰法对异丙基肾上腺素致大鼠心衰模型的实验研究  

The Experimental Study of TongYuHuaTan Methods on the Effects in Rat Model with Isoproterenol Induced Chronic Heart Failure

作　　者：王敏[1] 王晓峰[2] 艾静[3] 徐新利 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830054 [2]新疆医科大学附属中医院,新疆乌鲁木齐830000 [3]上海中医药大学附属曙光医院,上海200021 [4]新疆乌市第一人民医院分院,新疆乌鲁木齐830011

出　　处：《四川中医》2011年第4期23-25,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：上海市科委-国内合作(西部援助)项目(No.10495801500)

摘　　要：目的:研究通瘀化痰法对异丙基肾上腺素(ISO)诱导大鼠慢性心力衰竭(CHF)模型的治疗作用和对血清脑钠肽(BNP)的影响。方法:76只Wistar大鼠(雌雄各半)随机分为空白组和造模组,造模组皮下注射异丙基肾上腺素2次(170 mg.kg-1)间隔24小时,空白组皮下注射生理盐水0.25 ml,间隔24小时,6周后应用超声心动图检测,以左室射血分数≤45%确定造模成功,将心衰大鼠模型随机分为模型组、中药低、中、高剂量组及西药组,平均治疗4周后进行心脏超声检查,利用酶联免疫吸附法(ELISA)测定各组大鼠血清BNP的含量。结果:异丙肾致慢性心衰大鼠心功能下降、心脏重量指数增加、BNP升高明显;经治疗4周,各治疗组均有不同程度改善,其中中药中、高剂量组作用优于西药组,统计有显著性差异(P<0.01)。结论:通瘀化痰法能显著改善心功能,减轻心衰症状,逆转心室重构,其作用可能与抑制心衰大鼠血清BNP表达有关。Objective： To study the treatment of TongYuHuaTan Methods in rat model with isoproterenol induced chronic heart failure and to observe the effects on brain natriuretic peptide（BNP）.Methods： 76 Wistar rats（male and female） were randomly divided into blank control group and ISO-induced CHF group（ISO group）.The rats in ISO group were subcutaneously injected with 170 mg/kg ISO for 2 times at a 24 hours interval.While the rats in blank control group were given a subcutaneous injection of 0.25 ml normal saline for 2 times at a 24 hours interval.At the sixth week,echocardiography was used to evaluate the change of heart function in heart failure rats.The ISO-induced CHF rats,with an the left ventricular ejection fraction（LVEF）≤45%,were randomly divided into 5 groups： model group,the Traditional Chinese Medicine low,medium and high dose group and western medicine group;after 4 weeks of treatment,the heart function parameters were detected by echocardiogram,using enzyme-linked immunosorbent assay of serum BNP levels.Results： Isoproterenol induced renal decline in heart function in rats with chronic heart failure,heart weight index increased,BNP increased significantly;after treatment for 4 weeks,treatment groups improved in different degrees,including Traditional Chinese Medicine medium,the high dose group were superior to western medicine group,there was significant statistical differencec（P 0.01）.Conclusion： TongYuHuaTan Methods can significantly improve heart function,reduce heart failure symptoms,reverse ventricular remodeling.It＇s mechanism maybe relate to the expression of serum BNP.

关 键 词：通瘀化痰法 心力衰竭 大鼠 脑钠肽 异丙基肾上腺素 

分 类 号：R285.5[医药卫生—中药学]