大黄药材中大黄苷元含量测定  

Determination of Anthraquinones Aglycone in RADIX ET RHIZOMA RHEI

作　　者：宿爱山[1] 王淑美[1] 梁生旺[1] 李建生[2] 王战红[1] 

机构地区：[1]广东药学院,广东广州510006 [2]河南中医学院,河南郑州450008

出　　处：《安徽农业科学》2011年第11期6382-6383,6518,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(81073024);广东省自然科学基金项目(9451022401003860);广东药学院重点培养教师资助计划

摘　　要：[目的]采用HPLC法测定不同产地的大黄药材中5种蒽醌苷元的含量。[方法]HPLC色谱条件为:色谱柱为Diamonsil C18柱(5μm,250.0 mm×4.6 mm);流动相为甲醇-0.05%磷酸水溶液(V∶V=85∶15);流速为1 ml/min;检测波长245 nm;进样量为20μl。[结果]大黄各苷元线性关系良好,平均回收率为97.55%～100.87%,RSD值为1.38%～2.47%。大黄药材中各苷元含量范围分别为:芦荟大黄素0.26%～0.52%,大黄酸0.21%～1.47%,大黄素0.26%～0.40%,大黄酚0.29%～1.95%,大黄素甲醚0.48%～2.72%。[结论]该方法精密度良好、重现性、稳定性好,适合大黄药材中各大黄苷元的质量控制。[Objective] To determine contents of five anthraquinones aglycones by HPLC.[Method] HPLC conditions were as follows： Diamonsil C18 column（4.6 mm×250.0 mm,5 μm）,mobile phase of methanol-0.05% phosphoric acid（V∶ V=85∶ 15）,flow rate of 1 ml/min,detection wavelength of 245 nm and sampling amount of 20 μl.[Result] Good in linear relationship,the recovery rate of each aglycone ranged from 97.55%-100.87% with RSD value of 1.38%-2.47%.Contents of aloe-emodin,rhein,emodin,chrysophanol and physcion in RADIX ET RHIZOMA RHEI were： 0.26%-0.52%,0.21%-1.47%,0.26%-0.40%,0.29%-1.95% and 0.48%-2.72%,respectively.[Conclusion] Good in precison,repeatability and stability,the method established in this study favored the quality control of each aglycone in RADIX ET RHIZOMA RHEI.

关 键 词：大黄 蒽醌类苷元 含量测定 HPLC法 

分 类 号：S567[农业科学—中草药栽培]