奶牛体外受精相关影响因素分析  

Study on Influencing Factors In Vitro Fertilization of Dairy Cattle

作　　者：刘长彬[1] 倪建宏[1] 张宾[1] 卢守亮[1] 万鹏程[1] 石国庆[1] 

机构地区：[1]新疆农垦科学院新疆生产建设兵团绵羊繁育生物技术重点实验室,新疆石河子832000

出　　处：《安徽农业科学》2011年第11期6399-6401,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科学技术部"863课题"(2008AA101011);兵团科技攻关计划课题(2007ZX02);科技人员服务南疆专项(2010GG57)

摘　　要：[目的]探讨和研究奶牛体外受精(IVF)各技术环节中一些因素,对奶牛IVF整个过程的影响。[方法]研究不同来源的卵母细胞、精液的不同处理方法、不同的培养体系,不同受精时间,受精密度对奶牛IVF的影响。[结果]屠宰场采集的卵母细胞和活体采卵收集的卵母细胞的卵裂率和囊胚发育率差异不显著;对精子分别采用percoll梯度密度离心和上浮法处理,两组的卵裂率囊胚发育率无明显差异,用SOF液和BO液体外培养,两培养体系的卵裂率和囊胚发育率均差异不显著;受精时间为8、10 h及10、12 h的囊胚发育率均差异不显著,但受精8 h的囊胚发育率显著高于受精时间12 h;受精微滴(100μl)中放置成熟的卵母细胞数(20、40个),两组的卵裂率和囊胚发育率均下降,且差异显著。[结论]随受精时间的延长,卵裂率和囊胚发育率逐渐降低,差异不显著,但受精8 h的囊胚发育率显著高于受精12 h;受精微滴(100μl)中放置20个成熟的卵母细胞,可取得较好的体外受精效果。[Objective] The aim of this study was to analyze the different factors influencing the results of In Vitro Fertilization（IVF）in dairy cattle in order to establish an efficient IVF system.[Method] Influences of oocytes from different sources,different treatment ways of semen,different culture systems and different fertilization time and different fertilization density on IVF were summarized.[Result] Differences of cleavage rates and blastocyst rates of oocytes collected from slaughterhouse and live body were not significant.The sperms were treated by percoll and upriver.There were no significant differences at cleavage rates and blastocyst rates between the two groups.The cleavage rates and blastocyst rates in SOF and BO were not significantly different between the two groups.The blastocyst rate was not significantly different between 8 h and 10 h and between 10 h and 12 h.The former cleavage rates and blastocyst rates was higher than the latter.Putting 20 matured oocytes cells into 100 μl fertilization droppings lowered cleavage rates and blastocyst rates and the differences were significant.[Conclusion] Along with the prolonging of fertilization time,cleavage rates and blastocyst rates lowered gradually and differences between them were not significant.8 h fertilized blastocyst rate was higher than 12 h fertilization;putting 20 matured oocytes cells into 100 μl fertilization droppings could have better IVF effects.

关 键 词：奶牛 体外受精(IVF) 影响因素 

分 类 号：S814.3[农业科学—畜牧学]