总蛋白室间质评成绩不满意原因分析  

Analysis of dissatisfactory results of total protein in external quality assessment

作　　者：梁敏文[1] 邸玉玮[1] 戴耀宗[1] 黎艳湘[1] 李芳[1] 

机构地区：[1]广东省医学科学院广东省人民医院病理医学部检验科,广东广州510080

出　　处：《中国误诊学杂志》2011年第7期1515-1517,共3页Chinese Journal of Misdiagnostics

摘　　要：目的查找2009年第1次全国常规化学总蛋白(TP)室间质评(EQA)成绩不满意原因。方法用贝克曼LX20 MC(A组)、DXC800 MC(B组)及DXC800 CC(C组)仪器检测40份患者新鲜血清样本、三个厂家定值室内质控(IQC)血清和2009年第1次剩余EQA血清TP,数据采用One-Way ANOVA分析,新鲜血清样本同时依据EP9-A2文件方法分析,以CLIA’88规定的允许误差(EA)1/2为标准,判断三组仪器检测结果可比性。结果 A、B组检测EQA血清TP仍不满意(PT<80%),C组满意(PT=100%);三个厂家质控血清TP均在控,但MC(A/B组)与CC(C组)部分差异有统计学意义(P<0.05);患者新鲜血清样本差异无统计学意义(P>0.05),MC(A/B组)与CC(C组)相关性均良好(r2>0.95、P<0.01),系统误差(SE)小于1/2 EA。结论 TP EQA成绩不满意原因是EQA血清基质效应造成的。Objective To find the reason which led to the dissatisfactory results of the first total protein（TP） in external quality assessment（EQA） in 2009.Method TP concentrations of remained EQA serum,40 fresh serum specimens from clinical patients and internal quality control（IQC） serum from three companies were detected by BECKMAN LX20 MC（A group）,DXC800 MC（B group） and DXC800 CC（C group） respectively.Data was analyzed by one-way ANOVA.At the same time,part of data was analyzed by the way from EP9-A2.According to the standard of 1/2 permissible error（EA） of CL IA′88,the comparability of the three detection systems was judged.Results TP concentrations of EQA serum in A and B groups were still as before（PT80%）,in C group was good（PT=100%）.Though TP concentrations of IQC serum in A,B and C group are all in control,there was different between C and AConclusion The matrix effect of EQA serum in different detection systems may lead to errors which result in the isfactory results of TP in EQA.

关 键 词：血清学试验 

分 类 号：R446.11[医药卫生—诊断学]