子宫骶韧带成纤维细胞受力后胶原合成变化的初步研究  

Preliminary study on metabolism of collagen in uterus sacral ligament fibroblasts under stress

作　　者：李晓伟[1] 苗娅莉[1] 王建六[1] 魏丽惠[1] 蒋励[1] 

机构地区：[1]北京大学人民医院妇科,100044

出　　处：《中华妇产科杂志》2011年第3期172-176,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：卫生部临床学科重点项目（2007-2009）;首都医学发展科研基金（20072010）;高等学校博士学科点专项科研基金（20090001120083）

摘　　要：目的了解宫骶韧带成纤维细胞在应力情况下，I型胶原蛋白、Ⅲ型胶原蛋白、脯氨酰羟化酶（PH4）及基质金属蛋白酶1（MMP-1）基因表达的变化，以研究盆底功能障碍性疾病发病的生物力学特征。方法选取北京大学人民医院因子宫良性病变行开腹子宫切除术的8例患者，术中切取宫骶韧带0．8～1．0cm，以组织块法进行成纤维细胞的原代培养，对细胞进行加力（另设不加力的静力对照细胞），逆转录PCR技术检测I型胶原蛋白、Ⅲ型胶原蛋白、PH4及MMP-1mRNA的表达水平。结果应力持续24h，当应力为8％时，I型胶原蛋白（1．13±0．24）、Ⅲ型胶原蛋白（1．05±0．31）和PH4（1．11±0．31）mRNA的表达均较静力对照细胞（mRNA表达水平均为1）有增加趋势；当应力为4％和12％时，I型胶原蛋白（0．86±0．26和0．85±0．25）、Ⅲ型胶原蛋白（0．74±0．29和0．83±0．38）mRNA的表达均较静力对照细胞有减少趋势。应力持续1h之后去除，当应力为4％时，随去除后时间的延长，I型胶原蛋白（1d为0．79±0．40、2d为0．97±0．24、3d为1．46±0．75）、Ⅲ型胶原蛋白（1d为0．86±0．40、2d为0．99±0．60、3d为1．59±0．82）和PH4（1d为1．11±0．51、2d为1．17±0．54、3d为1．37±0．39）mRNA的表达水平逐渐上升，至撤力后3d达较高值；当应力为8％时，I型胶原蛋白（1d为1．16±0．62、2d为1．01±0．51、3d为1．05±0．80）mRNA的表达在1d时达较高值，随时间延长有下降趋势，而Ⅲ型胶原蛋白（1d为0．99±0．69、2d为1．59±0．55、3d为1．03±0．91）和PH4（1d为1．05±0．31、2d为1．07±0．80、3d为0．85±0．31）mRNA的表达在2d时达较高值，随后下降；当应力为4％和8％时随撤力时间变化，MMP-1mRNA的表达水平均在1d（分别为1．44±0．42、2．04±0．38）达到较高值。结论适当的应力可以促进盆底胶原的合成，应力过大或过小Objective To detect the expression of type I collagen, type IH collagen, prolyl-4- hydroxylases （PH4） and matrix metalloproteinase 1 （ MMP-1 ） in sacral ligament fibroblasts under stress, to understand the collagen synthesis and metabolism in stress situations change. Methods Eight patients who underwent abdominal hysterectomy for uterine benign disease were enrolled in this study. Primary sacral ligament fibroblasts were isolated by explant. After mechanical loading, gene expression of type I , llI collagen, PH4 and MMP-1 were measured. Results Stress of 8% continuing for 24 hours, collagen I （1.13±0.24）, collagen Ill （1.05 ±0.31） mRNA expression and PH4 expression （1.11 ±0.31） compared with static groups （1） showed increasing trends;when the stress were 4% and 12% , collagen I （0. 86± 0. 26 and 0. 85 ±0. 25 ）, collagen Ⅲ showed increasing trends （ 0.74 ± 0. 29 and 0. 83 ± 0. 38 ）mRNA expression were decreased. After removal of the stress, in the stress of 4% for 1 hour, collagen I （ 0. 79 ±0.40, 0. 97 ±0. 24 and 1.46± 0. 75 ）, collagen III （0. 86 ±0.40, 0. 99 ±0. 60 and 1.59 ±0. 82） and PH4 （1.11 ±0. 51, 1.17 ±0. 54 and 1.37±0. 39） mRNA expression increased gradually. In 8% stress group, collagen I mRNA expression （1.16 ± 0. 62, 1.01±0. 51 and 1.05 ± 0. 80） reached the peak in day 1, and collagen HI （0.99_＋0.69, 1.59_＋0.55 and 1.03 ＋0.91） and PH4 （1.05 ±0.31, 1.07 ± 0.80 and 0. 85 ±0. 31 ） mRNA expression reached the peak in day 2, then decreased. 4% and 8% of the stress with time after the change, MMP-1 mRNA expression have peaked at day 1. Conclusions Moderate stress could contribute to pelvic floor collagen synthesis, too much or too little stress is not conducive to the synthesis of collagen. Collagen I and collagen Ill on the stress response may be different, the former have faster reaction than the latter. PH4 were involved in the synthesis of collagen, while MMP-1 may play a role in collagen degradation.

关 键 词：子宫 骶骨 韧带 成纤维细胞 胶原 子宫脱垂 尿失禁 压力性 细 胞应力 

分 类 号：R711[医药卫生—妇产科学] R714[医药卫生—临床医学]