荧光素酶标的人肝癌细胞裸鼠异种移植瘤模型的生物发光成像和PET-CT成像比较  

Comparison between Bioluminescent Imaging and Small-animal PET-CT in Xenotransplantation Tumor Model with Luc-expressing Cell

作　　者：李小颖[1] 高凯[1] 董伟[1] 张连峰[1,2] 

机构地区：[1]中国医学科学院北京协和医学院实验动物研究所,卫生部人类疾病比较医学重点实验室,北京100021 [2]国家中医药局人类疾病动物模型3级实验室,北京100021

出　　处：《中国比较医学杂志》2011年第3期10-13,F0002,79,共6页Chinese Journal of Comparative Medicine

基　　金：卫生部项目,实验动物和人类疾病动物模型资源扩展(200802036);十一五新药专项支持(2008ZX09305-001)

摘　　要：目的利用荧光素酶基因标记的人肝癌细胞株BEL-7402建立裸鼠肝原位移植模型,及小鼠肝原位移植模型的生物发光和小动物PET-CT成像的比较。方法构建表达荧光素酶基因的真核表达载体并将其转入人肝癌细胞BEL-7402,经梯度浓度G418筛选获得稳定表达荧光素酶基因的细胞克隆并扩大培养。BALB/cA-nu裸鼠肝门静脉接种5×105个发光细胞使其成瘤,活体荧光成像和小动物PET-CT成像系统观察肿瘤的生长情况。结果获得了稳定表达Luc的人肝癌细胞株,将其接种到裸鼠体内,活体荧光成像系统观察发现能够成瘤,小动物PET-CT影像观察发现小鼠肝脏边缘对18 F-FDG有高摄取区域。结论利用荧光素酶基因标记的人肝癌细胞BEL-7402成功建立了原位肝癌裸鼠模型,小动物活体成像结合小动物PET-CT技术为原位肿瘤模型的建立提供了一种新的可靠的技术,为进一步研究肝癌生长转移机制和药物开发提供了新的有用工具。Objective To establish hepatic carcinoma mouse model with human BEL-7402 cells expressing luciferase and to compare bioluminescence imaging with that by small-animal PET-CT.Method The vector containing luciferase gene was constructed and transfected into human BEL-7402 liver tumor cell line and selected with G418 to obtain stable Luc-expressing clones.5 × 105 cells,constitutively expressing luciferase,were inoculated to liver of BALB /cA-nu through hepatic portal vein for assessment of tumor growth with the whole-body optical imaging system and small-animal PET-CT.Result The stable Luc-expressing BEL-7402 cell lines were abtained,which could grow to tumor mass after implantation.There was high uptake of 18 F-FDG at the edge of liver with small-animal PET-CT.Conclusion Luc-labeled BEL-7402 cell lines and a mouse tumor model based on the cell lines are established,the whole-body optical imaging system combined with small-animal PET-CT provides a new and reliable technology for the in situ tumor model,which is a new tool to further study the mechanism of metastasis and drug discovery.

关 键 词：人肝癌细胞 肿瘤模型 荧光素酶 生物发光成像 小动物PET-CT 

分 类 号：R541[医药卫生—心血管疾病] R332[医药卫生—内科学]