急性肺损伤大鼠肺组织基质金属蛋白酶2及其抑制因子蛋白和mRNA的表达  

Expression of the MMP-2 and TIMP-2 Protein and Its mRNA in Rats with Acute Lung Injury Caused by Lipopolysaccharide

作　　者：阮琼[1] 苏中昊[2] 杨爱东[2] 李文雯[2] 汪东颖[2] 吴中华[2] 庞慧芳[2] 

机构地区：[1]上海市第八人民医院急诊内科,上海200233 [2]上海中医药大学基础医学院,上海201203

出　　处：《中国比较医学杂志》2011年第3期39-42,81,共5页Chinese Journal of Comparative Medicine

基　　金：上海市教委高校高水平特色发展项目(No.(2005)81);上海高校选拔培养优秀青年教师科研专项基金(P13908)

摘　　要：目的探讨内毒素致急性肺损伤(ALI)大鼠肺组织核转录因子-κB(NF-κB)、基质金属蛋白酶2(MMP-2)及其抑制因子(TIMP-2)蛋白和mRNA表达的变化。方法 20只雄性Wistar大鼠随机分为2组:对照组、LPS模型组,每组再分为4 h和8 h两个亚组。尾静脉注射脂多糖(LPS)(10 mg/kg)建立大鼠急性肺损伤模型。检测血白细胞计数、支气管肺泡灌洗液(BALF)蛋白含量,采用免疫组化ABC法和实时荧光定量PCR分别测定肺组织NF-κB、MMP-2、TIMP-2蛋白及其mRNA的表达,并观察肺组织病理变化。结果与对照组相比,模型组4 h和8 h时大鼠肺组织中的NF-κB、MMP-2蛋白染色阳性面积率及其mRNA表达均显著增高(P<0.01)、TIMP-2蛋白染色阳性面积率及其mRNA表达均明显降低(P<0.05或P<0.01)。病理学观察显示,模型组大鼠肺组织出现出血及坏死。结论内毒素致急性肺损伤的发病机制可能与NF-κB、MMP-2蛋白及其mRNA表达升高、TIMP-2蛋白及其mRNA表达降低有关。Objective To investigate the changes of NF-κB,MMP-2 and TIMP-2 protein and its mRNA in the rats with acute lung injury（ALI） caused by lipopolysaccharide（LPS）.Methods Twenty male Wistar rats were randomly divided into two groups： normal control group,LPS model group.Each group had two subgroups,4 hours and 8 hours after LPS were injected.The ALI model was established by intravenous injection of LPS（10 mg /kg）through a tailvein.Then the white blood cell（WBC） in blood and the protein content in bronchial alveolar lavage fluid（BALF） were measured,the expressions of nuclear factor-κB（NF-κB）,matrix metalloproteinase-2（MMP-2） and tissue inhibitor of matrix metalloproteinases（TIMP-2） protein in pulmonary tissues were measured by immunohistochemistry ABC,the expression NF-κB,MMP-2 and TIMP-2mRNA were measured by real-time fluorescent polymerase chain reaction（PCR）,while the histopathology of the lung injury was observed by light microscope.Results Compared with normal group,the expression

关 键 词：脂多糖 急性肺损伤 NF-κB MMP-2 TIMP-2 

分 类 号：R563.9[医药卫生—呼吸系统] R332[医药卫生—内科学]