一种家兔持续静脉麻醉方法  

A Method of Persistent Intravenous Anesthesia for Rabbits

作　　者：孙婷婷[1] 王宏坤[2,3] 

机构地区：[1]福建医科大学附属协和医院,福州350001 [2]南京军区福州总医院,福州350025 [3]第四军医大学西北医院,西安710032

出　　处：《中国比较医学杂志》2011年第3期56-58,共3页Chinese Journal of Comparative Medicine

摘　　要：目的为进行某些特殊项目的实验研究,建立一种持续麻醉实验动物模型。方法戊巴比妥钠粉剂加生理盐水溶解,配成1%水剂,按3 mL/kg(30 mg/kg)耳缘静脉注射。诱导成功后约2 h经留置针使用恒速微量泵1 mL/h.kg给药,维持4 h后调节至2 mL/h.kg给药,并长期维持。每日上午、下午各停止静脉给药1 h,为动物饲食水。结果经实验证明家兔可耐受120 h连续麻醉。实验动物耐受情况好,家兔麻醉前呼吸48～68次/min,心跳140～200次/min,体温为39.4℃±1.0℃。以3～6 mg/s速度,成年兔的正常耳缘静脉注射后,平均3+2.8 s实验动物呈三度麻醉状态,无自主运动,未见兴奋躁动过程,呼吸24±6.6次/min。死亡率12.5%。持续麻醉期间动物二便正常。结论长期持续麻醉的效果要求是以避免动物挣扎的镇静为主,戊巴比妥适合于建立长期麻醉实验动物模型。该方法在爆炸伤和创面愈合等实验实际应用中效果理想。Objective To build up an ongoing experimental animal models of anesthesia for the purpose of special projects for the conduct of experimental studies is necessary.Method Pentobarbital powder was dissolved in saline into 1% liquid,and was injected by ear vein at 3 mL/kg.About 2 hours after the induction,the solution was given by constant-speed micro pump via intravenous trocar at 1 mL /（h.kg）,which lasted for 4 hours.Then the dosage was adjusted to and maintained for long term at 2 mL /（h.kg） body weight dose.Each day,the administration was suspended for an hour in both morning and afternoon,and meanwhile,the animals were fed with water.Resul As showed in the experiment,the rabbits tolerated 120 h continuous anesthesia,and were in good condition of tolerance.Before anesthetized,the rabbits’breath rate was 48 ～ 68 times/ min,heart rate was 140 ～ 200 times/min,body temperature was 39.4 ± 1.0℃.After ear vein injection at the speed of 3 ～ 6mg / sec,the rabbits were in drugged state every 3 ± 2.8 s,no voluntary movement or excited agitation was observed,and the breath rate was 24 ± 6.6 times/min.The mortality of these animals was 12.5%.During anesthesia,the animals kept normal urination and bowel movements.Conclusion The major requirement of long-term continuous anesthesia is that the animals should be under sedation and avoid struggling.Pentobarbital is suitable for establishment of animal models for long-term anesthesia.This method has a satisfactory effect on treatment of blast injury and wound healing in practical applications.

关 键 词：戊巴比妥 家兔 持续麻醉 

分 类 号：R826.5[医药卫生—临床医学]