外源脯氨酸对盐胁迫下甜瓜幼苗硝酸还原的影响  

Effects of Exogenous Proline on Nitrate Reduction in Melon Seedlings under Salt Stress

作　　者：颜志明[1,2] 孙锦[1] 郭世荣[1] 

机构地区：[1]南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095 [2]江苏农林职业技术学院,江苏句容212400

出　　处：《植物科学学报》2011年第1期118-123,共6页Plant Science Journal

基　　金：国家重点基础研究发展计划(2009CB119000);现代农业产业技术体系建设专项资金资助项目(nyhyzx10-07);国家科技支撑计划项目(2008BADA6B07);江苏省农业三项工程项目(SX(2008)026)

摘　　要：采用营养液水培方法,以"雪美"品种甜瓜(Cucumis melo L.)为材料,研究了外源脯氨酸(Proline)对盐胁迫下甜瓜幼苗叶片和根系硝酸还原的影响。结果表明:(1)盐胁迫提高了甜瓜幼苗叶片和根系内铵态氮(NH4+-N)和可溶性蛋白含量;降低了硝态氮(NO-3-N)含量和硝酸还原酶(nitrate reductase,NR)活性。(2)外源施用脯氨酸明显地提高了盐胁迫下甜瓜幼苗叶片和根系内NO-3-N和可溶性蛋白含量;降低了盐胁迫下甜瓜幼苗叶片和根系内NH+4-N含量;增强了盐胁迫下甜瓜幼苗体内NR活性。研究结果表明,外源脯氨酸可以通过调节甜瓜幼苗体内硝酸还原酶活性和氮化合物含量来缓解盐胁迫对甜瓜幼苗植株的伤害。Nutrient solution hydroponics was adopted to study the effects of exogenous proline on nitrogen reduction in the leaves and roots of ＂xuemei＂ melon（Cucumis melo L.） seedlings under NaCl stress.The results shown that：（1）Salt stress increased the NH4＋-N and soluble protein content in leaves and roots of melon seedlings;and decreased NO-3-N content and nitrate reductase（NR） activity;and（2）Exogenous proline increased NO-3-N and soluble protein content in leaves and roots of melon seedlings under salt stress;decreased NH4＋-N content;and increased NR activities.These results indicated that exogenous proline could ease injury suffer when melon seedling experience salt stress relying on adjusting NR activity and nitrogen compounds contents.

关 键 词：脯氨酸 盐胁迫 甜瓜幼苗 氮代谢 有机渗透调节物质 

分 类 号：Q945.78[生物学—植物学]