斑蝥酸钠对人胃癌裸鼠皮下移植瘤组织中血管生成作用的研究  

Effect of Sodium Cantharidinate on the Angiogenesis of Nude Mice with Human Gastric Cancer

作　　者：梁枫[1] 王明艳[2] 黄文斌[3] 李爱剑[1] 

机构地区：[1]安徽中医药高等专科学校基础教学部,安徽芜湖241000 [2]南京中医药大学基础医学院,江苏南京210046 [3]南京医科大学附属南京市第一医院病理科,江苏南京210006

出　　处：《中药材》2011年第3期343-346,共4页Journal of Chinese Medicinal Materials

基　　金：安徽省教育厅自然科学研究项目(KJ2007B201)

摘　　要：目的:研究斑蝥酸钠对人胃癌裸鼠移植瘤组织中血管生成的影响。方法:采用人胃癌BGC823细胞株种植裸鼠皮下建立人胃癌移植瘤模型。应用免疫组化法测定斑蝥酸钠对移植瘤组织中血管内皮生长因子(VEGF)及肿瘤内微血管密度(MVD)表达的影响。结果:中、高剂量斑蝥酸钠治疗组VEGF表达积分极显著低于低剂量组和对照组(P<0.01),中剂量组与高剂量组、低剂量组与对照组之间VEGF表达积分无差异(P>0.05)。中、高剂量斑蝥酸钠治疗组MVD极显著低于对照组和低剂量组(P<0.01),但中剂量组和高剂量组之间无显著性差异(P>0.05)。结论:斑蝥酸钠可能通过下调肿瘤细胞分泌VEGF,抑制肿瘤血管生成而抑制肿瘤细胞生长。Objective：To study the effect of sodium cantharidinate on the angiogenesis of nude mice with human gastric cancer.Methods：Nude mice xenograft models of human gastric cancer were established by injecting gastric carcinoma cell BGC823 into peritoneal.Expression of VEGF and MVD labeling by CD34 in human gastric cancer cells were measured by immunohistochemis-try.Results：Expression scores of VEGF in medium dose and high dose group with sodium cantharidinate treatment were lower than those in low dose and control group（P0.01）.There was no significant difference between medium dose and high dose group or low dose and control group（P0.05）.MVD values in medium and high dose group with sodium cantharidinate treatment were lower than those in low dose and control group（P0.01）,but there was no significant difference between medium dose and high dose group（P0.05）.Conclusions：sodium cantharidinate can inhibit the growth of the tumor by down-regulating VEGF expression of the tumour cell and the angiogenesis of the tumour.

关 键 词：斑蝥酸钠 胃癌 裸鼠移植瘤 血管内皮生长因子 微血管密度 

分 类 号：R285.5[医药卫生—中药学]