龟板提取物对PC12细胞凋亡的影响及其机制  

Effects and Mechanism of Plastrum testudinis Extracts on PC12 Apoptosis

作　　者：刘洋[1] 伍艺灵[1] 曹佳会[1] 陈东风[1] 周健洪[1] 邓汝东[1] 

机构地区：[1]广州中医药大学解剖学教研室,广东广州510405

出　　处：《中药材》2011年第3期400-403,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30772861)

摘　　要：目的:探讨龟板提取物(Plastrum testudinis Extracts,PTE)对6-羟基多巴胺(6-OHDA)诱导的PC12细胞凋亡的保护作用及其机制。方法:采用无血清培养PC12细胞同时100μmol/mL 6-OHDA损伤24 h的方法建立PC12细胞凋亡模型。细胞分为正常对照组、6-OHDA组及PTE 3、30μg/mL组四组。在施加处理因素24 h后,MTT比色分析测定细胞光密度值,Ammexin V/PI双染流式细胞术测定细胞凋亡率,Western blot检测BCL-X/L的表达水平。用Bio-Rad Quantity One凝胶分析系统对条带进行半定量分析。结果:MTT与流式细胞术结果显示PTE能提高PC12细胞活力,降低PC12细胞凋亡率,并呈剂量依赖性,PTE 3、30μg/mL组与模型组比较,差异具有统计学意义。Western blot结果显示龟板提取物使BCL-X/L表达增强,并呈剂量依赖性。结论:龟板提取物具有抑制6-羟基多巴胺诱导PC12细胞凋亡的作用,其作用机制可能与上调BCL-X/L的表达有关。Objective：To observe the inhibitive effects of Plastrum testudinis Extracts（PTE） on 6-Hydroxydopamine（6-OHDA）induced PC12 cells apoptosis and explore its mechanism.Methods：PC12 apoptosis model was established by serum starvation and damaged for 24 hours.The cells were randomly divided into four groups：control group,6-OHDA group,PTE 3,30 μg/mL group.Cell optical density was determined by MTT;Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry（FCM）,and Western blot was applied to detect the BCL-X/L expression.Results：MTT and FCM analysis demonstrated that PTE can elevate PC12 cells viability and reduce their apoptotic ratio in a dose dependent manner. Western blot showed that PTE promoted the expression of BCL-X/L. Conclusion：PTE can inhibit the apoptosis of PC12 induced by 6-OHDA in a dose dependent manner,and its mechanism maybe associated partially with up-regulating BCL-X/L signaling pathway.

关 键 词：龟板提取物 BCL-X/L PC12细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]