卫星搭载高油与普通玉米自交系变异性观察  

VARIABILITY INDUCED BY SPACEFLIGHT ENVIRONMENT ON HIGH OIL AND NORMAL MAIZE LINES

作　　者：徐小炜[1] 徐丽[1] 董昕[1] 金危危 陈绍江[1] 

机构地区：[1]中国农业大学国家玉米改良中心/北京作物遗传改良重点实验室,北京100193

出　　处：《核农学报》2011年第2期220-225,共6页Journal of Nuclear Agricultural Sciences

基　　金："十一五"国家科技支撑计划(2008BAD97B03)

摘　　要：以经卫星搭载的高油玉米自交系BY815和常规自交系1145、F349为材料,从中筛选突变体并对其表型,分子生物学和细胞学变异特点进行研究。结果表明:在SP1代,BY815出现表型变异的频率为21.61%,而1145和F349出现表型变异的频率仅为2.57%和3.13%。在SP2代仅发现6个表型变异突变体,其中,BY815白化苗突变体HT-3和1145叶色条纹状斑点突变体HT-5较有特点。在这2个突变体中,正常植株与突变体植株的比率均表现为3∶1,符合孟德尔单基因遗传规律。对这6个突变体材料进行细胞学观察,未发现染色体的异常行为。利用130对SSR引物分析表明,只有1个来自1145的突变体有8.46%的多态性差异位点,而在其余突变体中未发现差异位点。High oil inbred line BY815 and two normal inbred lines 1145 and F349 treated with spaceflight were used for variability analysis.Results showed that the mutation rate of BY815 was 21.61% in SP1,while the mutation rates of 1145 and F349 were 2.57% and 3.13% respectively.Only six mutants were found from these three materials in SP2,of which two mutants,HT-3 from BY815 exhibiting albino leaf color and HT-5 from 1145 exhibiting stripe-like spots leaves,were worthy of further study.Genetic analysis of the two mutants showed that the segregation ratio of normal and mutant phenotypes was 3 ∶ 1,which was in accordance with Mendel’s single gene inheritance law.Cytological observation of all the six mutants showed no chromosome abnormalities.By using SSR（simple sequence repeat） method,130 pairs of primers were employed and only one mutant originated from inbred line 1145 showed polymorphic and the mutated loci rate of the genome in this mutant was 8.46%.

关 键 词：玉米 卫星搭载 突变体 SSR 

分 类 号：S513[农业科学—作物学]