空间诱变后甜椒叶片光合、色素和超微结构的变化  

CHANGE OF PHOTOSYTHETIC,PIGMENT AND ULSTRUCTURE OF SWEET PEPPER LEAVES AFTER SPACE INDUCTION

作　　者：郭亚华[1] 谢立波[1] 孟凡娟[2] 刘录祥[3] 王雪[1] 高永利[1] 周宇[1] 庞洪影[2] 

机构地区：[1]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040 [3]中国农业科学院作物科学研究所/国家农作物航天诱变技术改良中心,北京100081

出　　处：《核农学报》2011年第2期237-241,共5页Journal of Nuclear Agricultural Sciences

基　　金：航天育种工程项目(2009HT2000);农业部农业公益性行业科研专项(200803034);黑龙江省自然基金重点项目(ZD200818-01);黑龙江省自然基金项目(C200943);东北林业大学研究生创新基金项目

摘　　要：为探索空间条件对植物的诱变机理,以经空间诱变的甜椒(Capsicum frutescens Linn.)第4代植株叶片为主要试验材料,研究其叶片光合指标、色素含量和叶片细胞叶绿体超微结构的变化特点。结果表明:空间诱变后连续4代自交授粉获得的变异体SP06-31植株叶片内,叶绿素a、b,叶绿素a与b的比值和总叶绿素以及类胡萝卜素的含量与对照L06-30相比均有所下降,而且叶绿素a含量和总叶绿素含量与对照L06-30相比差异显著。L06-30和SP06-31的光合速率及蒸腾速率、气孔导度的变化表现为"单峰型";胞间二氧化碳浓度的变化表现为"漏斗型"。同时,叶片细胞出现细胞破裂、叶绿体内淀粉粒数量增多、细胞间隙增大、细胞基质以及叶绿体外渗及部分叶绿体基粒片层模糊等现象。The M4 sweet pepper after space induction was used to study the mechanism of space mutagenesis.The changes in photosynthetic indexes,pigment content and ultrastructure of chloroplast in sweet pepper were studied.The results indicated that the contents of leaf chlorophyll a,b,the ratio of a and b,total chlorophyll and carotenoid content decreased in SP06-31,a mutant compared to those of the control L06-30,The contents of chlorophyll a and total chlorophyll were significantly difference from those of control.The change of photosyn the tic rate of both L06-30 and SP06-31showed a one-peak pattern,and the change of transpiration rate showed one-peak pattern,too.The change of intercellular CO2 concentration showed a funnel-like trend and that of stomatal conductance was followed by a one-peak trend.The mesophyll cells were broken,the number of starch grains in chloroplast was ncreased,intercellular space was enlarged,and parts of chloroplast grana lamella were ambiguity in the mutant.

关 键 词：空间诱变 甜椒 光合 色素 超微结构 

分 类 号：S641.3[农业科学—蔬菜学]