电子束辐照对灰葡萄孢菌分生孢子萌发活性及致病力的影响  

EFFECT OF ELECTRON BEAM IRRADIATION ON CONIDIAL GERMINATION ACTIVITY AND PATHOGENICITY OF Botrytis cinerea

作　　者：张婷[1,2] 陈召亮[2] 乔勇进[1,2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海市农业科学院农产品保鲜加工研究中心,上海201106

出　　处：《核农学报》2011年第2期286-291,共6页Journal of Nuclear Agricultural Sciences

基　　金：上海市科技兴农重点项目(沪农科攻字(2008)第10-6号);上海市科委国际合作计划项目(073907003)

摘　　要：以灰葡萄孢病菌(Botrytis cinerea)分生孢子为研究对象,采用0.5、1.0、2.0和3.0kGy剂量电子束辐照灰葡萄孢分生孢子,分别测定5℃和25℃培养条件下辐照对灰葡萄孢分生孢子的萌发活性及致病力的影响。结果表明:25℃和5℃培养条件下,2.0kGy处理分生孢子完全萌发时间比对照分别延迟了5和9d;24h分生孢子萌发率分别降低了46.57%和33.68%;芽管长度抑制率分别为25.12%和74.29%;分生孢子对草莓的致病力显著降低,25℃培养2d时,经2.0kGy辐照处理后的分生孢子病情指数为4.17;5℃培养15d时,病情指数仅为15.28,显著低于对照。电子束处理可显著抑制灰葡萄孢菌分生孢子的萌发及芽管伸长,延迟其萌发时间,降低其致病力,且剂量越高,效果越明显。Conidia of Botrytis cinerea were irradiated by electron beam at 0.5,1.0,2.0 and 3.0kGy.The influence of electron beam on the activities of conidial germination and pathogenicity at the temperatures of 5℃ and 25℃ were tested,respectively.The results showed that the electron beam could inhibit germination of conidia and the length of germ tube of Botrytis cinerea,and delay the germination time.It could also decrease the pathogenicity obviously and higher irradiation dose showed stronger effects.Compared with control,the complete germination time of conidia extended to 5 and 9d at the cultivate temperatures of 25℃ and 5℃,after 2kGy of irradiation,and the germination rate was reduced 46.57% and 33.68%,respectively.The inhibition rates of germ tube were 25.12% and 74.29% when cultured 24h.The pathogenicity of Botrytis cinerae to strawberry was reduced significantly.After 2.0kGy irradiation and cultivate at 25℃ for 2d,the disease index was 4.17 and it decreased to 15.28 after cultivation of 5℃ for 15d.Electron beam treatment could inhibit the spore germination and germ tube elongation of Botrytis cinerea significantly,delayed the germination time,and reduced its pathogenicity,the higher the dose,the effect was more obvious.

关 键 词：电子束 灰葡萄孢菌 分生孢子 萌发 致病力 

分 类 号：S432.44[农业科学—植物病理学]