酒精耐受性枯草芽孢杆菌发酵马铃薯薯渣的研究  

Studies on Fermentation Condition of Potato Residues by Alcohol Tolerance Bacillus subtilis

作　　者：张向东[1] 杨谦[1] 宋佳 范金霞[1] 

机构地区：[1]哈尔滨工业大学生命科学系,哈尔滨150001

出　　处：《吉林农业大学学报》2011年第2期181-184,共4页Journal of Jilin Agricultural University

基　　金："十一五"国家科技支撑计划项目(2006BAD07A01);国家"863"计划项目(2006AA10Z424)

摘　　要：从大庆盐碱土中分离到1株高浓度酒精耐受性纤维素降解细菌菌株ZY62。对该菌株的16sRNA进行序列分析确定其为枯草芽胞杆菌(Bacillus subtilis)。以马铃薯渣和汁水作为发酵培养基初步优化ZY62菌株产羧甲基纤维素(CMC)酶条件,试验结果为pH值自然、37℃、150 r/min发酵时CMC酶活最高;发酵120 h后,薯渣与汁水转化获得的发酵产物中单细胞蛋白含量达到18.5%。小鼠增重试验结果表明马铃薯渣与汁水发酵后产物可部分替代豆粕。An alcohol tolerance efficient cellulose-degrading bacterium ZY62 was isolated.The bacterium was proven Bacillus subtilis by 16sRNA sequence analysis.The conditions of CMC enzyme production were optimized when fermented in potato residues and juice without any foreign substances.When B.subtilis was inoculated at 37 ？℃,150 r/min for 120 h without pH adjustment,the quality of single cell protein reached 18.5% of total fermentation product.The fermented product can partly replace soybean meal of the same weight in mouse feed test.The single cell protein from potato residues can be wildly used as feed in the future.

关 键 词：枯草芽孢杆菌 马铃薯薯渣 筛选鉴定 酒精耐受性 

分 类 号：S816.4[农业科学—饲料科学]