灵芝孢子油对四氯化碳诱导的小鼠肝损伤的保护作用  

Protective effects of the oil from spores of Ganoderma lucidum on carbon tetrachloride-induced hepatic injury in mice

作　　者：朴日龙[1] 崔永 许青松[3] 

机构地区：[1]延边大学基础医学院机能实验中心,吉林延吉133002 [2]延边圣霖菌业有限公司,吉林延吉133002 [3]延边大学基础医学院生理学与病理生理学教研部,吉林延吉133002

出　　处：《延边大学医学学报》2011年第1期20-22,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]探讨长白山地产灵芝孢子油对四氯化碳所致化学性肝损伤模型小鼠肝脏的保护作用.[方法]取18~22 g昆明种小鼠,雌雄各半,随机分为5个组,即空白对照组、模型对照组、灵芝孢子油大（1.00 g/kg）、中（0.33 g/kg）、小（0.17 g/kg）剂量组,连续灌胃给药2周.第14天隔夜禁食16 h后,腹腔注射给予各给药组和模型对照组小鼠0.125%（体积分数）四氯化碳0.01 mL/g,腹腔注射给予空白对照组等剂量的玉米油,4 h后正常灌胃,24 h后处死,解剖取出肝脏,测定肝组织中天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性.[结果]模型对照组AST,ALT活性与空白对照组比较显著降低（P〈0.01）,灵芝孢子油小、中、大剂量组AST,ALT活性与模型对照组比较均显著升高（P〈0.05,P〈0.01）.[结论]长白山地产灵芝孢子油对四氯化碳所致小鼠化学性肝损伤具有一定的保护作用.OBJECTIVE To investigate the protective effects of the oil from spores of Ganoderma lucidum（OSCGL） on carbon tetrachloride（CCl4）-induced hepatic injury in mice.METHODS18～22g of mice of Kunming species were randomly divided into the control,model,and small,middle and large dosage of OSCGL（1.00,0.33,0.17g/kg BW） groups.Mice of each group were administrated by gastric gavage of OSCGL for two weeks.At 14 days,model and OSCGL groups were injected in peritoneal cavity with 0.125% of CCl4（0.0lmL/g）,and the control group was injected with the same volume of corn oil.After 4h of injection,the mice were administrated by gastric gavage normally,and after 24h,the mice were sacrificed and performed autopsy to tate out of livers,and activity of AST and ALT in liver were detected 24h after gavage.RESULTSThe activity of AST and ALT were significantly lower in model group than in control group（P0.01）,and were significantly higher in small,middle and large dosage of OSCGL than in model group（P0.05,P0.01）.CONCLUSIONThe OSCGL has the protective effects on CCl4-induced hepatic injury in mice.

关 键 词：肝疾病 灵芝 四氯化碳 小鼠 

分 类 号：R285.5[医药卫生—中药学]