转基因番茄防龋疫苗急性和亚慢性毒性试验  

Study on Acute and Subchronic Toxicity of Transgenic Tomato Anti-caries Vaccine

作　　者：白国辉[1] 刘建国[1] 田源[1] 陈筑 韩琦[1] 白朋元[1] 顾瑜[1] 管晓燕[1] 

机构地区：[1]遵义医学院附属口腔医院,贵州遵义563000 [2]贵阳市口腔医院,贵州贵阳550000

出　　处：《贵州农业科学》2011年第4期146-149,共4页Guizhou Agricultural Sciences

基　　金：国家自然基金资助项目"转基因植物可食融合体防龋疫苗的研究"(30160086);贵州省优秀科技人才省长专项基金资助项目"转基因植物防龋疫苗生态安全性的初步研究"[黔省专合字(2006)44];贵州省科技攻关项目"转基因植物防龋疫苗的免疫效果和安全性研究"[黔科合NY字(2006)3043];贵阳市科学技术计划项目"嵌合体转基因植物防龋疫苗的免疫原性和安全性研究"[2008筑科农合同字第28-6号]

摘　　要：为评价表达致龋变异链球菌表面蛋白和霍乱毒素B亚单位的转基因番茄防龋疫苗的安全性,进行了大鼠经口灌胃染毒试验。结果表明,急性毒性试验中大鼠均未见毒性症状,急性毒性LD50>20 g/kg;亚慢性毒性试验中大鼠均未见毒性症状,在血液学和生化、脏器系数和组织病理学检查中,试验组大鼠与阴性对照组间均无显著差异(P>0.05)。说明,表达致龋变异链球菌表面蛋白和霍乱毒素B亚单位嵌合体蛋白的转基因番茄防龋疫苗对大鼠安全。The healthy rats were perfused with transgenic tomato juice containing PAcA/CTB protein and PAcP/CTB protein to evaluate the safety of transgenic tomato anti-caries vaccine with surface protein of streptococcus mutans and cholera toxin B subunit.The results showed that the toxic symptom was not found on tested rats in the acute and subchronic toxicity tests,the LD50 of acute toxicity was more than 20g/kg,there were no significant difference in hematological index,blood biochemistry index,organ coefficient and histopathology between the tested group and negative CK group,which indicates that transgenic tomato anti-caries vaccine with surface protein of streptococcus mutans and cholera toxin B subunit is safe to rats.

关 键 词：转基因番茄 防龋疫苗 急性毒性 亚慢性毒性 龋病 变异链球菌 

分 类 号：S641.2[农业科学—蔬菜学]