姜黄素对椎板切除术后大鼠硬膜外瘢痕组织中羟脯氨酸含量的影响  

Effect of curcumin on content of hydroxyproline in the epidural scar tissue of rats after laminectomy

作　　者：张伟[1] 王立新[2] 

机构地区：[1]蚌埠医学院,安徽蚌埠233030 [2]江苏省连云港市第二人民医院骨科,222023

出　　处：《蚌埠医学院学报》2011年第4期332-334,共3页Journal of Bengbu Medical College

摘　　要：目的:观察姜黄素对椎板切除术后大鼠硬膜外瘢痕组织中羟脯氨酸含量的影响,探讨其作用机制。方法:36只SD大鼠手术切除L1椎板后造成0.3 cm×0.5 cm硬脊膜裸露区,随机分为3组。空白对照组(A组)不作任何治疗;胶原蛋白海绵组(B组)、姜黄素胶原蛋白海绵组(C组)分别在椎板缺损处放置0.5 cm×0.7 cm胶原蛋白海绵和吸收姜黄素二甲基亚砜溶液(0.25 g/ml)的胶原蛋白海绵。术后4周进行瘢痕组织羟脯氨酸含量测定,并按Rydell法大体评定粘连程度。结果:B、C组硬膜外瘢痕组织中羟脯氨酸含量均低于A组(P<0.05和P<0.01)。Rydell分级显示,C组与A组差异有统计学意义(P<0.01),而B组与A组差异无统计学意义(P>0.05)。结论:姜黄素能减少椎板切除术后硬膜外瘢痕生成,抑制胶原纤维的形成。Objective：To observe and evaluate the effect of curcumin on content of hydroxyproline in the epidural scar tissue of rats after laminectomy,and to explore its mechanism.Methods：Thirty-six SD rats with 0.3 cm×0.5 cm epidural exposed area after L1 laminectomy surgery were randomly divided into 3 groups.The control group（group A） received no treatment;the collagen sponge group（group B） and the curcumin collagen sponge group（group C） were respectively placed 0.5 cm×0.7 cm collagen sponge and collagen sponge absorbing curcumin dimethyl sulfoxide solution（0.25 g/ml） in the laminectomy defect.Four weeks later,the level of hydroxyproline in the scar tissue was determined,and the degree of peridural adhesion was generally evaluated according to the Rydell standard.Results：The content of hydroxyproline in group B and group C was lower than that in group A（P0.05 and P0.01）.Conclusions：Curcumin can reduce epidural scar production and inhibit the formation of collagen fibers.

关 键 词：椎板切除术 姜黄素 硬膜外瘢痕增生 羟脯氨酸 

分 类 号：R687.3[医药卫生—骨科学]