茉莉花愈伤组织诱导及悬浮细胞培养  

Jasminum sambac Callus Induction and Suspension Cell Culture

作　　者：庄哲煌[1] 蔡汉权[1] 陈丹生[1] 

机构地区：[1]韩山师范学院生物系,广东潮州521041

出　　处：《长江蔬菜》2011年第8期21-24,共4页Journal of Changjiang Vegetables

基　　金：韩山师范学院重点科研项目(2005-413545)

摘　　要：以茉莉花(Jasminum sambac)花瓣为外植体在不同培养基上诱导出愈伤组织,通过对其类型的筛选从中选择疏松愈伤组织进行悬浮培养后,初步建立悬浮细胞培养系。结果表明,不同激素可诱导出疏松型(CA)、泥状型(CB)和绿色块状型(CC)愈伤组织,其分化方向也不同。用CA型和CB型愈伤组织均能建立悬浮细胞培养系,其中以泥状愈伤组织为起始材料较佳,大约11 d可建立初步的悬浮细胞培养系。Petals of Jasminum sambac were induced and cultured callus on the media with different phytohormones concentration.The preliminary stable suspension culture system was established after choosing the loose callus from different types for cell suspension culture.The results indicated that different phytohormones could form three types of callus,CA,CB and CC which had diverse polarization direction.Both the CA and CB callus could be used as initial materials for establishing suspensions,and it took about 11 days to establish cell suspensions from the better callus,CB callus.

关 键 词：茉莉花 愈伤组织 悬浮细胞培养 

分 类 号：S685.16[农业科学—观赏园艺]