基于纳米多孔金(NPG)电极的电化学DNA传感器的研制  

Preparation of DNA Electrochemical Sensor Based on NPG Electrodes

作　　者：胡孔诚[1] 朱英杰[1] 吕庆春[2] 刘艳辉[2] 兰东晓[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042 [2]国家海洋局天津海水淡化与综合利用研究所,天津300192

出　　处：《青岛科技大学学报（自然科学版）》2011年第2期128-132,137,共6页Journal of Qingdao University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金项目(21075072);生命分析化学教育部重点实验室开放课题(KLACLS08015)

摘　　要：研制了基于纳米多孔金(NPG)电极的电化学DNA传感器。首先用脱合金法制备多孔纳米金(NPG),然后将其修饰到玻碳电极上制备NPG电极。在该电极上固定捕获DNA,与靶DNA杂交后,以邻菲咯啉钴为指示剂,用脉冲伏安法对目标DNA进行检测。DNA/NPG传感器与裸金电极制备的同类型传感器相比,具有更高的灵敏度。并对DNA/NPG传感器的选择性和再生性进行了研究。Electrochemical DNA sensor based on the nanoparticles porous gold（NPG） electrode was prepared.Firstly NPG was prepared using a dissolved alloy method.The product was modified with a glassy carbon electrode.DNA was fixed and captured in the electrode.After target DNA hybridization,with the [Co（phen）33＋] as an indicator,pulse voltammetry was used to detect the target DNA.Results showed that NPG electrochemical DNA sensor has a higher sensitivity than gold electrode sensor,and it also has a good selectivity and reproducibility.

关 键 词：DNA传感器 NPG电极 修饰电极 

分 类 号：O657.1[理学—分析化学]