浑善达克沙漠褐沙蒿根际细菌组成及其季节性变化  

COMMUNITY COMPOSITION AND SEASONAL VARIATION OF RHIZOBACTERIA OF Artemisia intramongolica IN DESERT HUNSADERK

作　　者：冯福应[1] 刘发来[1] 李蘅[1] 孙丽鹏[1] 孟建宇[1] 秦玉英 

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物实验所,呼和浩特010018 [2]内蒙古蒙牛乳业集团股份有限公司,呼和浩特011500

出　　处：《内蒙古农业大学学报（自然科学版）》2011年第1期157-161,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学(30860015);内蒙古自治区自然科学(2010BS0506);内蒙古自治区重大自然科学(2009ZD03);内蒙古自治区高等学校科学(NJ10054)

摘　　要：植物根际细菌对植物的生长和健康具有非常积极的作用,尤其是对处于营养贫瘠等逆境下的沙漠植物,研究细菌菌群组成是理解植物生长促进根际细菌(PGPR)与植物互作关系并应用于提高植物生产力的前提和基础。基于16S rDNA基因的文库构建和分析,本文对比分析了浑善达克沙漠中褐沙蒿根际细菌群组成及其随季节的变化情况,探讨了PGPR在褐沙蒿生长发育过程中的作用和功能。结果表明:浑善达克沙漠中的褐沙蒿根际细菌类群主要有10类,即Firmicutes、Verrucomicrobia、Gemmatimonadetes、Acidobacteria、Actinobacteria、Bacteroidetes、Chloroflexi和α-、β-、δ-proteobacteria;在褐沙蒿生命活动相对旺盛的4月份和8月份最优势的类群是属于α-proteobacteria的具有固氮功能的细菌和具有降解大分子物质并兼有生防功能的Sphingomonadales菌群;而在褐沙蒿代谢相对弱的时期(11月份),根际细菌多样性反而高于植物代谢强的时期(4月份和8月份)。本研究提示我们,在挖掘PGPR资源时应该注意选择合适的植物生长时期。Rhizobaeteria significantly and positively affect growth and health of the associated plant, especially for plant struggling under stress like poor nutrition in desert, and the study therefore on bacterial composition is the basis for application of plant growth promoting rhizobacteria （PGPR） in improving plant productivity and understanding their interaction. Analysis and construction of library based on 16S rDNA gene showed that, the dominant rhizobacteria of Arternisia intramongolica sampled from Desert of Hunsaderk were totally fell into 10 groups, including Firmicutes , Verrucomicrobia , Gemmatimonadetes , Acidobacteria , Actinobacteria , Bacteroidetes , Chloroflexi ,and α- ,β- ,δ-proteobacteria. Bacteria with nitrogen -fixing belonging to α -proteobacteria predominated in April and, the bacteria of Sphingomonadales with the ability for maeromolecule degrading and phytopathogen suppressing were the majority in August, along with the actively metabolizing of the plants in both stages; but with the reduced metabolism of the plants in November, the bacterial diversity higher than that in April and August with robust plant metabolization. These results suggested that the suitable stage of plant should be considered extensively in exploration for PGPR.

关 键 词：褐沙蒿 根际细菌 菌群结构 16S RDNA 

分 类 号：S917.1[农业科学—水产科学]