金银忍冬茎和果实中绿原酸的提取及含量测定  

EXTRACTION AND DETERMINATION OF CHLOROGENIC ACID IN Lonicera maackii STEM AND FRUIT

作　　者：青梅[1] 王素巍[1] 杨慧[1] 李娟[1] 多露[1] 

机构地区：[1]内蒙古医学院药学院,呼和浩特010059

出　　处：《内蒙古农业大学学报（自然科学版）》2011年第1期281-284,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

摘　　要：目的:为忍冬属植物中绿原酸的提取及含量测定寻求更适合的方法。方法:采用超声技术提取金银忍冬茎、果实中的绿原酸,高效液相色谱法测定绿原酸的含量,色谱柱为C-18柱,流动相为甲醇-0.4%磷酸(35:65),流速为1mL/min,检测波长为327nm。结果:仪器精密度良好(RSD=1.41%),测定方法具有较好的重现性试验(RSD=1.56%),供试品溶液在8h内基本稳定(RSD=1.70%)。绿原酸在1.06μg-6.36μg范围内与峰面积具有良好线性关系(R2=0.999 4),平均回收率为98.79%(RSD=1.65%)。金银忍冬茎和果实中绿原酸的含量分别为0.796%,0.379 8%。结论:超声提取时间缩短到15m in,流动相中用甲醇-0.4%磷酸代替了文献中的乙腈-0.4%磷酸,为忍冬属植物中绿原酸的提取及含量测定提供了更为经济、便捷的方法。Objective： To seek for more suitable methods of extraction and determination of Chlorogenic Acid from Lonicera. Methods ： clorogenic acid （CHA） was extracted from stem and fruit of Lonicera maackii with Ultrasonic wave and determined by HPLC on a C - 18 column, methanol - 0.4% H3PO4 （35 ： 65） as a mobile phase, 1 mL/min of flow rate, detection wavelength at 360 nm. Results ： Good precision （ RSD = 1.41% ） and repeatability （ RSD = 1.56% ） were obtained. The sample was stable within 8 hours （ RSD = 1. 70% ）. The linear range of chlorogenic acid was 1.06 - 6. 36μg （ R2 = 0.9994）. And the average recovery was 98.79% （ RSD = 1. 65% ）. CHA content of chlorogenic acid in stem and fruit of Lonicera maacki were 0. 796% , 0.3？98% respectively. Conclusions ： Ultrasonic time was shorten to 15min, the flowing ratio was methanol -0.4% phosphoric acid replaced acetonitrile -0.4% reported in literature. This study offered more economic and convenient method of CHA extraction and determination in Lonicera.

关 键 词：金银忍冬 绿原酸 超声提取 高效液相 含量测定 

分 类 号：Q946.829[生物学—植物学]