黄芪注射液对小鼠同种皮肤移植存活率的影响  

EFFECT OF ASTRAGALUS INJECTION ON THE SURVIVAL OF THE ALLOGRAFTS OF SKIN IN MICE

作　　者：于新娟[1] 侯桂华[2] 朱新红[1] 林存智 

机构地区：[1]青岛市市立医院,山东青岛266071 [2]山东大学实验核医学研究所 [3]青岛市胸科医院

出　　处：《青岛大学医学院学报》2011年第2期119-122,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨黄芪注射液对小鼠同种皮肤移植存活率的影响及对脾细胞糖皮质激素受体（GR）与血清皮质醇的调节作用。方法建立小鼠同种皮肤移植模型,通过腹腔注射给予黄芪注射液和环磷酰胺（CTX）,观察黄芪注射液对同种移植物生存期的影响;采用受体放射配基结合分析和放射免疫分析方法,观察移植后不同时间小鼠脾细胞GR及血清皮质醇的改变以及黄芪注射液和环磷酰胺对其的影响。结果与对照组相比,黄芪组、黄芪＋CTX组及CTX组移植皮片存活时间明显延长,差异有显著性（F=48.58,q=5.11~14.48,P〈0.05）。对照组移植后第14天GR结合位点数明显降低,与未移植组比较,差异有显著性（F=48.44,q=10.95,P〈0.05）;CTX组GR结合位点数进一步降低,与对照组比较,差异有显著性（q=4.22,P〈0.05）;黄芪组GR结合位点数升高至正常水平,与对照组比较,差异有显著性（q=11.79,P〈0.05）,与未移植组比较,差异无显著性（q=0.84,P〉0.05）;黄芪与CTX合用后逆转CTX对GR结合位点数的影响（q=10.72,P〈0.05）。对照组移植后第14天血清皮质醇水平明显升高,与未移植组比较,差异有显著性（F=29.46,q=7.64,P〈0.05）;CTX组血清皮质醇水平降低,与对照组比较,差异有显著性（q=3.46,P〈0.05）;黄芪组、黄芪＋CTX组血清皮质醇水平升高,与对照组比较,差异有显著性（q=4.54、5.22,P〈0.05）。结论黄芪注射液可以明显抑制同种移植排斥反应,提高移植物存活率,其作用机制可能与上调GR数量和血清皮质醇水平,从而使内源性糖皮质激素更好地发挥作用有关。Objective To study the effect of astragalus injection on the survival of the skin allografts in mice and its mechanism. Methods A mouse model of alloskin grafting was created.Astragalus injection and CTX were administered intraperitoneally.The effect of astragalus on survival of the graft was observed.With radioligand banding assay of receptors（RBA）,the quantity of GR in the animal spleen cells at different times before and after the transplantation and after administration of astragalus injection and CTX were detected.Cortisol concentration in serum was examined via radioimmunoassay（RIA）. ResultsCompared with that of the controls,the survival duration of the allografts in mice treated with astragalus alone,astragalus and CTX,and CTX alone were prolonged significantly（F=48.58;q=5.11-14.48;P0.05）.The amount of GR in spleen cells in control group decreased significantly in day 14 after transplantation vs non-transplanted（F=48.44;q=10.95;P0.05）,and the amount of GR in CTX group decreased more than the control（q=4.22,P0.05）.Astragalus increased GR level to normal level,compared with the control,the different was significant（q=11.79,P0.05）;but not significant vs non-transplanted（q=0.84,P0.05）.Astragalus＋CTX reversed the low GR level of CTX（q=10.72,P0.05）.The cortisol concentration of the control group rose markedly on day 14 after transplantation vs the non-transplanted（F=29.46;q=7.64;P0.05）,while that of CTX group decreased vs control（q=3.46,P0.05）.Astragalus alone and Astragalus＋CTX enhanced the serum cortisol concentration vs the control（q=4.54,5.22;P0.05）. Conclusion Astragalus injection can inhibit the rejection of the allograft and prolong its survival.The mechanisim might be associated with upregulating the amount of GR and serum cortisol,and increase the action of endogenous glucocorticoid.

关 键 词：黄芪注射液 环磷酰胺 受体 糖皮质激素 同种皮肤移植 小鼠 近交BALBC 

分 类 号：R285.5[医药卫生—中药学]