IRTK与妊娠期糖尿病胰岛素抵抗的关系  

RELATIONSHIP BETWEEN INSULIN RECEPTOR TYROSINE KINASE OF THE SKELETAL MUSCLE AND INSULIN RESISTANCE OF WOMEN WITH GESTATIONAL DIABETES MELLITUS

作　　者：张宁[1] 叶元华[1] 徐金娥[1] 匡国芳[1] 

机构地区：[1]青岛大学医学院附属医院产科,山东青岛266003

出　　处：《青岛大学医学院学报》2011年第2期123-125,128,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨骨骼肌组织胰岛素受体酪氨酸激酶（IRTK）活性与妊娠期糖尿病（GDM）胰岛素抵抗的关系。方法采用ELISA法测定了30例GDM病人（GDM组）、30例糖耐量正常孕妇（正常妊娠组）及20例糖耐量正常非孕妇女（对照组）胰岛素体外刺激前、后骨骼肌组织IRTK活性。采用葡萄糖氧化酶法及放射免疫法测定空腹血葡萄糖（FPG）及空腹血胰岛素（FINS）水平,计算胰岛素抵抗指数（HOMA-IR）。结果 GDM组FPG、FINS、HOMA-IR均明显高于正常妊娠组（F=21.7~40.2,q=5.42~10.56,P〈0.01）;正常妊娠组FINS、HOMA-IR明显高于对照组（q=3.76、4.36,P〈0.01）。胰岛素刺激前各组骨骼肌组织IRTK活性比较,差异均无显著性（P〉0.05）;胰岛素刺激后IRTK活性均升高,但GDM组明显低于正常妊娠组（F=95.0,q=10.95,P〈0.01）,正常妊娠组明显低于对照组（q=9.52,P〈0.01）。GDM组HOMA-IR与胰岛素刺激后骨骼肌组织IRTK活性呈负相关（r=-0.625,P〈0.01）。结论骨骼肌组织IRTK活性下降可能是GDM病人胰岛素抵抗的分子机制之一。Objective To investigate the relationship between insulin receptor tyrosine kinase（IRTK） of the skeletal muscle and insulin resistance of the women with gestational diabetes mellitus（GDM）. Methods The activity of IRTK of skeletal muscle was determined by ELISA in 30 patients with GDM（GDM group）,30 normal pregnant women（normal-pregnancy group） and 20 non-pregnant women（control group）.Fasting plasma glucose（FPG） and fasting insulin（FINS） were measured by oxidized assay and immunoradioassay.Insulin resistant index（HOMA-IR） was calculated according to the data of FPG and FINS.Results The levels of FPG,FINS and HOMA-IR of the GDM group were significantly higher than that of the normal-pregnancy group（F=21.7-40.2,q=5.42-10.56,P0.01）.The levels of FINS and HOMA-IR of the normal pregnancy group were significantly higher than that of the control group（q=3.76,4.36;P0.01）.Before insulin stimulation,no significant differences were found among the three groups in terms of the IRTK activities（P0.05）;After insulin stimulation,the IRTK activities increased in all the subjects,but the IRTK activities of the GDM group were significantly lower than that of the normal-pregnancy group（F=95.0,q=10.95,P0.01）;while that of the normal-pregnant group was lower than that of the control（q=9.52,P0.01）.HOMA-IR of the GDM group was negatively correlated with the IRTK activity after insulin stimulation（r=-0.625,P0.01）. Conclusion Decreased insulin receptor tyrosine kinase of the skeletal muscle may be one of the molecular mechanisms of the insulin resistance in women with GDM.

关 键 词：糖尿病 妊娠 胰岛素抗药性 受体 胰岛素 酪氨酸激酶 

分 类 号：R714.256[医药卫生—妇产科学]