荧光定量PCR技术在植物病原检测方面的应用  

Application of Real-time PCR on detection of plant pathogen

作　　者：王义鹏[1,2] 许艳丽[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]中国科学院东北地理与农业生态研究所,哈尔滨150081

出　　处：《大豆科技》2011年第2期9-12,共4页Soybean Science & Technology

基　　金：黑龙江省科技计划项目"大豆胞囊线虫病综合防治技术研究"(GA09B103-10);国家自然科学基金项目"连作大豆胞囊线虫病抑制性土壤特性与发生机制"(30971900)

摘　　要：就实时荧光定量PCR的原理、应用和不足及前景等方面进行了论述。实时荧光定量PCR技术以其快速、灵敏、准确和高通量等特点受到了人们越来越多的重视,被广泛的应用于生物学研究的各个领域。近年来在植物病原检测方面,已建立了一大批植物病原的实时荧光定量PCR方法,这为植物病害的准确诊断与防控提供了强有力的工具。Real-time PCR principle,application,deficiencies and its prospect were introduced.More and more attentions were paid to Real-time PCR because of its rapidity,sensitivity and high-throughout.The technology was applied in different areas on biological researches.Los of detective methods on detection of plant pathogen using real-time PCR were established and great technical supplies were given to diagnosis,prevention and control of plant diseases.

关 键 词：实时荧光定量 植物病害 检测 

分 类 号：Q503[生物学—生物化学]