拟南芥微管蛋白TUA2基因表达载体的构建及亚细胞定位  

Onstruction of expression vector and subcellular localization of microtubulin TUA2 gene from Arabidopsis thaliana

作　　者：刘海浩[1] 吴立柱 潘延云[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2011年第2期40-42,47,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(30570993);河北省自然基金项目(C2008000292)

摘　　要：本研究以拟南芥(Arabidopsis thaliana.Columbia ecotype)为材料,运用PCR方法从其基因组中扩增得到了TUA2基因的序列,将其连接到表达载体GV3101上,构建成双元载体。把该质粒用基因枪转化法转化洋葱表皮,激光共聚焦显微镜观察显示,TUA2基因在细胞膜上表达。该结果为进一步研究TUA2的作用机理提供了基础。Genome sequence of Arabidopsis thaliana TUA2 genes has been amplificated by PCR,and was connected into the expression vector GV3101.The recombinant plasmid was transformed into onion epidermis by using gene gun.Laser confocal microscope revealed that the TUA2 gene expression in the cell membrane.This result provided the basis for further research of the function of TUA2.

关 键 词：拟南芥 TUA 基因枪 

分 类 号：Q257[生物学—细胞生物学]