胡黄连苷Ⅱ对大鼠脑缺血再灌注损伤后炎症反应影响  

EFFECT OF PICROSIDEⅡ ON INFLAMMATION REACTION AFTER CEREBRAL ISCHEMIA REPERFUSION INJURY IN RATS

作　　者：王岭[1] 孙丽[1] 高焕民[1] 王超[1] 孙锋[1] 

机构地区：[1]青岛大学医学院第二附属医院神经科,山东青岛266042

出　　处：《青岛大学医学院学报》2011年第1期62-64,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的研究胡黄连苷Ⅱ对大鼠脑缺血再灌注损伤后炎症反应的抑制作用。方法应用线栓法建立大鼠大脑中动脉闭塞再灌注模型,随机分为假手术组、模型组和治疗组,治疗组在缺血后2 h以微量注射器经尾静脉给予胡黄连苷Ⅱ(10 mg/kg)250μL,模型组和假手术组同步给予0.1 mol/L PBS 250μL。采用BEDERSON评分方法评价大鼠神经行为功能,采用氯化三苯基四氮唑染色观察大鼠脑梗死体积,采用原位TUNEL染色方法检测大鼠神经细胞凋亡情况,采用免疫荧光染色和Western Blotting方法检测大鼠脑组织基质金属蛋白酶-9(MMP-9)的表达。结果假手术组大鼠未出现行为功能异常,细胞凋亡数量较少,MMP-9表达较弱。模型组大鼠表现为神经行为功能障碍,缺血侧皮质和纹状体区出现梗死病灶,细胞凋亡数量增多,MMP-9表达增强。应用胡黄连苷Ⅱ干预治疗后,MMP-9表达较模型组显著降低,细胞凋亡数量明显减少,脑梗死体积显著减小,神经行为功能显著改善。结论胡黄连苷Ⅱ可抑制大鼠脑缺血再灌注损伤后炎症反应,对大鼠脑缺血再灌注损伤起到保护作用。Objective To investigate the inhibitory effect of picrosideⅡon inflammatory reaction after cerebral ischemic reperfusion injury in rats.Methods Rat models of middle cerebral artery occlusion reperfusion were created by intraluminal thread method and divided into three groups in random.The rats in the treatment group received an injection of picrosideⅡ（10 mg/kg） via tail vein 2 hours after the ischemic reperfusion,while those in the sham-operation group and model group received sameamount of 0.1 mol/L PBS instead.The neurological behavioral function was evaluated by BEDERSON＇s test and the volume of cerebral infarction measured after tetrazolium chloride（TTC） staining.The apoptotic cells were counted by TUNEL assay and the expression of matrix metalloproteinase-9（MMP-9） in brain tissue was determined by immunofluoresce and Western Blotting assay.Results Abnormal neurological behavioral function was not found in rats of sham operation group with few apoptotic cells and weak expression of MMP-9.Significant neurological functional disorder was noticed in rats of the model group,together with cerebral infarction appeared in ischemic cortex and striatum,the amount of apoptosis increased,and the expression of MMP-9 enhanced.After picrosideⅡ treatment,the neuronal apoptosis and expression of MMP-9 significantly reduced,the neurological function improved and infarction volume decreased when compared with those in the control group.Conclusion PicrosideⅡ could inhibit the inflammation reaction against cerebral ischemia and reperfusion injury in rats.

关 键 词：胡黄连苷Ⅱ 再灌注损伤 细胞凋亡 明胶酶B 大鼠 Wistar 

分 类 号：R285.5[医药卫生—中药学]