Optimization of Genetic Transformation System of Tobacco K326 Mediated by Agrobacterium  

根癌农杆菌介导烟草K326遗传转化体系优化(英文)

作　　者：赵勤[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《Agricultural Science & Technology》2011年第1期62-64,共3页农业科学与技术（英文版）

摘　　要：[Objective]The aim was to optimize genetic transformation system in tobacco K326 mediated by Agrobacterium.[Method]The leaf of tobacco aseptic seedling was taken as explants to study the optimization of Agrobacterium-mediated genetic transformation system.[Result] The highest transformation efficiency was obtained when the explants were pre-cultured in the medium of MS ＋ 2 mg/L 6-BA ＋ 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101（OD600 =0.6） for 5 min.The PCR detection proved that npt II gene had been integrated into the regenerated tobacco plants.[Conclusion]A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.[目的]优化根癌农杆菌介导烟草K326遗传转化体系。[方法]以烟草无菌苗的叶片为外植体,通过农杆菌介导法对其遗传转化体系进行了优化研究。[结果]外植体在MS+2mg/L6-BA+0.2mg/LIAA培养基上进行2d预培养后,用农杆菌GV3101(浸染浓度为OD600=0.6)浸染5min,转化效率最高,达63%;经过PCR检测初步证明nptII基因已整合到烟草再生植株中。[结论]该研究建立了高效烟草K326叶片农杆菌介导的遗传转化体系。

关 键 词：Agrobacterium tumefaciens TOBACCO Genetic transformation 

分 类 号：S572[农业科学—烟草工业]