Mg^(2+)对猪冠脉平滑肌细胞钙激活钾通道的激活作用的初步研究  

ACTIVATION OF CA^(2+)-ACTIVATED K^+ CHANNEL IN PORCINE CORONARY ARTERIAL SMOOTH MUSCLE CELLS BY MG^(2+)

作　　者：贺军[1] 曾晓荣[1] 杨艳[1] 刘智飞[1] 周文[1] 李建军[1] 

机构地区：[1]泸州医学院心血管疾病研究所心肌电研究室,646000

出　　处：《泸州医学院学报》1999年第3期194-196,共3页Journal of Luzhou Medical College

基　　金：卫生部优秀青年基金

摘　　要：目的：研究Mg2+对猪冠脉平滑肌细胞钙激活钾通道的作用，以揭示Mg2+扩血管作用的电生理机制。方法：应用膜片钳制技术inside－out方式记录冠脉平滑肌上的单通道活动，经PCLAMP软件进行微机采样储存数据和数据的分析处理。结果：Mg2+对猪冠脉平滑肌钙激活钾通道具有明显激活作用：增强通道开放概率P0，增加膜片通道开放数目。结论：Mg2+对钙激活钾通道的激活作用可能为Mg2+调节心血管活动的重要机制之一。Objective: Effects of Mg2+ on Ca2+ - activated K+ channel (Kca) in porcine coronary arterial smooth muscle cells were studied to reveal the electrophysiological mechanism about the effects of mg2+ on the dilatation of blood vessel. Methods: Inside-out method of patch clamp technique was used to record the activity of single channel, configurational application of PCLAMP-software for data sampling storing and analysis. Results: After magnesium was applied to the cytoplasmic face of the channels, activation of Mg2+ on Kca was observed: it increased dose-dependently their open probability(Po) and the numbers of open Ietels of channel in a patch. Conclusions: The activation of Mg2+on Bkca channel may be important in its regulation of cardiovasclar system.

关 键 词：钙激活钾通道 冠脉平滑肌细胞 膜片钳 镁离子 

分 类 号：R543[医药卫生—心血管疾病] R331.38[医药卫生—内科学]